1 / 25

Specificity of Antibodies: A Simulation

Specificity of Antibodies: A Simulation. The Patient: Figaro. Antibody Review. What type of macromolecule is an antibody? What shape is a typical antibody?. Protein. Antibody Review. What is an antigen? How many antigens can the antibody below bind to?. Foreign Molecule.

hiett
Download Presentation

Specificity of Antibodies: A Simulation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Specificity of Antibodies: A Simulation The Patient: Figaro

  2. Antibody Review • What type of macromolecule is an antibody? • What shape is a typical antibody? Protein

  3. Antibody Review • What is an antigen? • How many antigens can the antibody below bind to? Foreign Molecule

  4. A day in Figaro's Life

  5. Figaro Possible Antigens

  6. Ways to Test for Allergies Brain storm ways to test for possible allergies.

  7. The Antibody Simulation • Label 3 Petri Plates with initials, date, period, LB Agar, and TRIAL 1, 2 or 3 (depending on the plate).

  8. The Antibody Simulation • Cut the bottom off a large transfer pipet.

  9. The Antibody Simulation • Apply a slight suction by compressing the bulb of the pipet between your fingers.

  10. The Antibody Simulation • Use the transfer pipet to poke holes in the agar. • Make 4 holes around the edge of the agar.

  11. The Antibody Simulation • Make 1 hole in the center of the agar. 1 2 C 3 4

  12. Allergen 1: ? Allergen 2: ? Allergen 3: ? Allergen 4: ? The Antibody Simulation • In your notebook, draw a Petri plate and label which antigen goes into each outside hole. 1 2 3 4

  13. Figaro’s Blood Serum: Antibodies The Antibody Simulation • On your Petri plate drawing in your notebook, label the center hole as Figaro’s Blood Serum. C

  14. The Antibody Simulation • Use a sterile 1 mL pipet to add antigen/ antibody solution to each hole in the agar.

  15. The Antibody Simulation • Add enough antigen serum so the solution is at the top of the agar hole.

  16. The Antibody Simulation • Use a different sterile 1 mL pipet for each antigen solution.

  17. The Antibody Simulation • Fill the center hole with Figaro’s Blood Serum (antibody solution).

  18. The Antibody Simulation • Repeat these steps for all 3 trials/ Petri plates. • Cover all 3 Petri plates and leave undisturbed for 24 hours.

  19. Data Collection • Collect Petri plates. • Draw Petri plate results in your notebook. • Write down observations • Form a data table in Excel. • Create numeric scale to convert observations to data. • Collect data from each trial. • Average the data from trials.

  20. Precipitin Lines The Antibody Simulation: Results • A precipitin line will appear between one or more of the allergens and the blood serum.

  21. The Antibody Simulation: Results • Record results of each of the 3 trials using a numeric scale • 5 = strong precipitation; 0 = no precipitation • Calculate average results for each antigen

  22. Weak Allergy Strong Allergy The Antibody Simulation: Results • The brighter precipitin lines, the stronger the allergy.

  23. Agglutination = Precipitin Lines The Antibody Simulation: Results • This lab is an example of an Ouchterlony test. • When antibodies diffuse in agar and bind with antigens, it causes agglutination, or a white precipitin line.

  24. The Antibody Simulation: Results • Make an individual data table in Google documents. • On the Smart Board, add your group’s data to the table and make a class average data table on your Google spreadsheet below your individual data table.

  25. Antibody Simulation: Conclusion • Post your individual data table, class average data table, and your REE PE PA three-paragraph conclusion on a new page for this lab on your Google Site. • Results - Go back to the purposes of the lab. • Evidence - Numerical evidence. • Explanation - Go back to your hypothesis. • Possible Errors • 2 examples of errors • Avoid future errors • Practical Applications • Suggestions to patient/family. • Further testing • Value of this lab/test

More Related