Genetic Mapping in a Three-Point Test Cross

1. Genetic Mapping in a Three-Point Test Cross

15. sc x ec 12 14 14 16 56/1008 x 100 = 5.6 mu

16. ec vg 235 ec + 243 ec vg 016 ec + 014 + + 241 + vg 233 + + 014+ vg 012 506 502

17. sc vg 235 sc + 243 sc vg 012 sc + 014 + + 241 + vg 233 + + 014+ vg 016 502 506

18. --|-----------------------------------------------|--- sc 5.5 mu ec --------------------------|------------------------- vg

20. sc x ec 25 29 54/982 x 100 = 5.5 mu

21. ec x cv 44 37 81/982 x 100 = 8.2 mu

22. sc x cv 25 44 29 37 135/982 x 100 = 13.7 mu

23. --|-----------------------------------------------|--- sc 5.5 mu ec --|-----------------------------------------------|--- cv 8.2 mu ec --|-----------------------------------------------|--- sc 13.7 mu cv

24. 5.5 mu ec 8.2 mu --|-----------------|-----------------------------|--- sc 13.7 mu cv

26. cv x ct 45 03 40 05 93/1448 x 100 = 6.4 mu

27. ct x v 89 05 94 03 191/1448 x 100 = 13.2 mu

28. cv x v 45 89 40 94 268/1448 x 100 = 18.5 mu

29. --|-----------------------------------------------|--- cv 6.4 mu ct --|-----------------------------------------------|--- ct 13.2 mu v --|-----------------------------------------------|--- cv 18.5 mu v

30. 6.4 mu ct 13.2 mu --|-------------|--------------------------------|--- cv 18.5 mu v

31. 6.4 + 13.2 = 18.5 6.4 + 13.2 = 19.6 DOUBLE CROSSOVERS! /

37. cv x v 45 89 40 94 16 (5 + 3 = 8 X 2= 16) 284/1448 x 100 = 19.6 mu

38. 6.4 mu ct 13.2 mu --|-------------|--------------------------------|--- cv 19.6 mu v