DETECTION AND SURVEILLANCE

1. DETECTION AND SURVEILLANCE Sheila Grant Department of Biological Engineering UMC

2. Goal is to ensure that early and accurate detection is available for important pathogens and zoonotic pathogens in various environments and deployment mechanisms Goal

3. Methods of Introduction • Aerosol release • Food supply • Water supply • Direct infection • Direct exposure from infected people/animals

4. Biological sensors Field RT-PCR Syndromic surveillance How to Protect?

5. Introduction of FAD Sensors: “Detect” Syndromic: “Increase vigilance” Field PCR: “Increased surveillance” Provisional containmentmeasures implemented yes yes Detect? Detect? no no Laboratory confirmation Implement response measures Continue surveillance Integration of Surveillance Mechanisms

6. Biological detection elements and transducer system Microfabrication (Aerosol collection) Signal processing, transmission, and networking Modeling Sensor Development

7. Biological Detectors Transducers + • antibodies • peptides • receptors • optical • acoustic wave • electrochemical Biological detection element and transducer systems Biosensor system = bioterrorist agents

8. l2 lo DD l1 Protein A FRET Immunosensor • measures the conformational changes that occurs • when antibodies bind to select agents • technique can eliminate false positives since only • viable agents can elicit a • conformational • change.

9. Sensing Peptides Mechanical: Shear Horizontal-SAW (SH-SAW) biosensors will detect enzymes in an aqueous solution. This device will detect a change in wave propagation speed as the targeted enzyme in solution cleaves a specific peptide-construct, vastly increasing specificity. Optical: Additionally, labeled peptide-constructs can be immobilized to gold nanoparticles, which effectively quenches fluorescence. Upon interactions with target enzymes, the peptide is cleaved and fluorescence is enhanced.

10. Au nanoparticle SNARE Microsphere doped with Erbium at the surface Au nanoparticles spoil Q-factor Upconversion is inhibited 980 nm laser Upon SNARE cleavage, Au particles are released Upconversion is possible to detect Ring resonator toxin sensor using fluorescence method

11. Microfabrication and Nanotechnology Nanoporous waveguide materials Peristaltic Micro-pumps

12. Anodic Bonding between the two substrates Output Reservoir Excitation Window Detector WasteChamber Light guide Light guide Micro channel with a Liquid core wave-guide Excitation source Water PDMS Input Reservoir Meandering Type Micro-mixer Nanoporous Silica Analyte solution being pumped in Inline Detection using liquid core wave-guide (LCW) Cross Detection using solid core wave-guide Signal detection on a chip

13. Integrated Fluorescence Assay on a Chip Short light pulses are generated by the laser and directed onto the sensor fluorophore inside the flowcell.

14. Real time detection Centralized data based system Modeling Future directions

15. Xudong (Sherman) Fan Frank Feng Shubhra Gangopadhyay Kevin Gillis Mark Haidekker Susan Lever Darcy Lichlyter Graduate Students Shantanu Bhattacharya Rosalynn Manor Mary Pierce (now employed by MRI) Lisa Boettcher Acknowledgements