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A modular positive feedback-based gene amplifier

A modular positive feedback-based gene amplifier. Goutam J Nistala , Kang Wu, Christopher V Rao , Kaustbh D. Bhalerao Presented by Raven Reddy and Colin Reisterer March 9, 2011. Positive Feedback. Gene product enhances its own production Amplification Bistability Hysteresis

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A modular positive feedback-based gene amplifier

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  1. A modular positive feedback-based gene amplifier Goutam J Nistala, Kang Wu, Christopher V Rao, Kaustbh D. Bhalerao Presented by Raven Reddy and Colin Reisterer March 9, 2011

  2. Positive Feedback • Gene product enhances its own production • Amplification • Bistability • Hysteresis • Oscillation

  3. Constitutively Active LuxR

  4. Positive Feedback Amplifier Input: LuxRΔ Output: LuxRΔ, GFP

  5. One Component System Input: aTc Output: LuxRΔ, GFP

  6. One Component System

  7. One Component System Time Dependent Dosing Conditions With Postive Feedback Without Postive Feedback

  8. One Component System Tried to create “memory”

  9. Two Component System Input: Aspartate Output: LuxRΔ, GFP

  10. Two Component System Sensor output

  11. Conclusions • Proved modularity of LuxRΔ positive feedback system • Application to impedance matching? • Future goal: better predictability • Transient signal?

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