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Hematopoeisis

Hematopoeisis. VIBS 443/602. 113. Peripheral blood smear (May-Grunwald-Giemsa). 110. Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils. 19761. Bone marrow 19761. Bone marrow smear; low power view, showing negative images of fat globules.

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Hematopoeisis

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  1. Hematopoeisis VIBS 443/602

  2. 113 Peripheral blood smear (May-Grunwald-Giemsa)

  3. 110 Peripheral blood smear (Leishman-Giemsa)eosinophil, basophil, and neutrophils

  4. 19761 Bone marrow 19761

  5. Bone marrow smear; low power view, showing negative images of fat globules

  6. Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA • Nuclei of megakaryocyte • Red blood cell

  7. Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation. • Macrophage • Lymphocyte • Neutrophil

  8. Pronormoblast • Basophilic normoblast • Polychromatophilic normoblast • Orthochromatic normoblast • Myelocyte • Late metamyelocytes

  9. Pronormoblast • Basophilic normoblast • Polychromatophilic normoblast • Orthochromatic normoblast • Myelocyte • Late metamyelocytes

  10. Late promyelocyte • Late myelocyte/early metamyelocyte • Nearly mature PMN

  11. Smudge cell • Promyelocyte • Myelocyte • Metamyelocytes

  12. Pronormoblast • Metamyelocytes • Band cell

  13. Bone marrow: EM 12a • Reticular cell • Developing red blood cell

  14. Bone marrow; EM 8a • Lymphocyte • PNM • Red blood cell • eosinophil

  15. Bone marrow; EM 12b • PMN • Immature neutrophil • Red blood cell

  16. Routine H&E of bone • Marrow spicules • Lipid in fat cells

  17. H&E of bone marrow 1. megakaryocytes

  18. 19761

  19. H&E of bone marrow: • Red blood cell

  20. 420 Rib

  21. 420 Rib

  22. 421 Tibia, fetal

  23. 32583

  24. 32583

  25. 112 Red bone marrow smear (May-Grunwald-Giemsa)

  26. Cells of Erythropoisesis Pronormoblast round nucleus and thin rim of deep blue cytoplasm Basophilic normoblast nuclear condensation ("fractured nucleus") with a deep blue cytoplasm

  27. Polychromatophilic normoblast cytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red Orthochromatic normoblast must have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully condensed

  28. Reticulocyte no nucleus, mature cytoplasm with blue tint Red blood cell typical of mature cell seen in circulation

  29. Cells of Granulopoiesis Promyelocyte oval nucleus with deep blue cytoplasm and azurophilic granules Myelocyte cytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape

  30. Metamyelocyte deeply indented nucleus with nearly mature cytoplasm (no longer blue) Band cell nucleus just prior to lobulation with fully mature cytoplasm Neutrophil (mature) lobulated nucleus with fully mature cytoplasm

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