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Cystic Fibrosis From Gene to Protein

Cystic Fibrosis From Gene to Protein. Class instructions. In this lesson. Look at the normal and mutated CFTR gene Make messenger RNA (mRNA) from DNA- transcription Make protein from mRNA- translation. The devil is in the detail!.

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Cystic Fibrosis From Gene to Protein

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  1. Cystic FibrosisFrom Gene to Protein Class instructions

  2. In this lesson • Look at the normal and mutated CFTR gene • Make messenger RNA (mRNA) from DNA- transcription • Make protein from mRNA- translation

  3. The devil is in the detail! • The 5’ prime and 3’ prime ends of the bases must be round the right way! IMPORTANT: Do not take bases apart!!!

  4. Correct base pairing is critical! • Green (Guanine) pairs with yellow (Cytosine) • Blue (Adenine) pairs with orange (Thymine)

  5. Lesson – part 1 Normal CFTR Gene ΔF508 CF Mutation Sense Sense Antisense Antisense

  6. Transcription – Step 1

  7. Base pairing in mRNA- use brown sugars • Green (Guanine) pairs with yellow (Cytosine) • Blue (Adenine) pairs with light blue (Uracil)

  8. Transcription – Step 2 • To make an mRNA, you must use the antisense (red sugars) strand as as the template as base pairing is complimentary.

  9. Resulting mRNA Normal CFTR mRNA ΔF508 CFTR mRNA

  10. Translation • Turns mRNA into protein • Use transfer RNA (tRNA) which are attached to amino acids as intermediate • Amino acids have a direction too! N-terminal C-terminal

  11. Transfer RNA UAA Isoleucine CAA Valine CCA Glycine AAA Phenylalanine UAA Isoleucine tRNA – holds an anticodon at one end, and an amino acid at the other

  12. Using mRNA in 5’-3’ orientation, translate into protein.

  13. Separate tRNA & protein from mRNA

  14. Resulting protein Normal CFTR protein ΔF508 CFTR protein Isoleucine – Phenylalanine - Glycine Isoleucine – Glycine - Valine

  15. Polymerase Chain Reaction • Using the original chromosomes, separate strands • Find where primers stick to each strand

  16. Genetic Test • Using the original chromosomes, see what a PCR product will look like. • Remember: Each individual will have 2 chromosomes - one inherited from mother and one from father.

  17. DNA gel genetic test result

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