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Cell Membrane & Cell Transport

Cell Membrane & Cell Transport. What does the cell membrane look like?. It consists of 2 layers of lipids with their tails pointed inward. These lipids are called phospholipids . Their heads are hydrophilic (attracted to water) and tails are hydrophobic (repel water).

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Cell Membrane & Cell Transport

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  1. Cell Membrane & Cell Transport

  2. What does the cell membrane look like? • It consists of 2 layers of lipids with their tails pointed inward. These lipids are called phospholipids. Their heads are hydrophilic (attracted to water) and tails are hydrophobic (repel water).

  3. Photograph of a Cell Membrane

  4. What does the cell membrane look like? • It also contains several different types of proteins, carbohydrate markers, and cholesterol.

  5. Types of Cell Transport • Passive Transport- this type of transport requires NO energy because particles travel from where they are highly concentrated to a low concentrated area. They travel DOWN the concentration gradient. HI LO

  6. Types of Cell Transport Active Transport- this type of transport doesrequire energy. Particles travel from low concentration to high concentration. In other words, they move AGAINST or UP the concentration gradient. HI LO

  7. Simple Diffusion • Requires NO energy • Molecules move from area of HIGH to LOW concentration

  8. DIFFUSION Diffusion is a PASSIVE process which means no energy is used to make the molecules move, they have a natural KINETIC ENERGY

  9. Diffusion of Liquids

  10. Diffusion through a Membrane Cell membrane Solute moves DOWN concentration gradient (HIGH to LOW)

  11. Osmosis • Diffusion of water across a membrane • Moves from HIGH water potential (low solute) to LOW water potential (high solute) Diffusion across a membrane Semipermeable membrane

  12. Diffusion of H2O Across A Membrane High H2O potentialLow solute concentration Low H2O potentialHigh solute concentration

  13. Cell in Isotonic Solution 10% NaCL90% H2O ENVIRONMENT CELL NO NET MOVEMENT 10% NaCL 90% H2O What is the direction of water movement? equilibrium The cell is at _______________.

  14. Cell in Hypotonic Solution 10% NaCL90% H2O CELL 20% NaCL 80% H2O What is the direction of water movement?

  15. Cell in Hypertonic Solution 15% NaCL85% H2O ENVIRONMENT CELL 5% NaCL 95% H2O What is the direction of water movement?

  16. Cells in Solutions

  17. Isotonic Solution Hypotonic Solution Hypertonic Solution NO NET MOVEMENT OF H2O (equal amounts entering & leaving) CYTOLYSIS PLASMOLYSIS

  18. Cytolysis & Plasmolysis Plasmolysis Cytolysis

  19. Osmosis in Red Blood Cells Isotonic Hypertonic Hypotonic

  20. hypotonic hypertonic isotonic hypertonic isotonic hypotonic

  21. Three Forms of Transport Across the Membrane

  22. Passive Transport • Simple Diffusion • Doesn’t require energy • Moves high to low concentration • Example: Oxygen or water diffusing into a cell and carbon dioxide diffusing out.

  23. Passive Transport • Facilitated diffusion • Doesn’t require energy • Uses transport proteins to move high to low concentration • Examples: Glucose or amino acids moving from blood into a cell.

  24. Proteins Are Critical to Membrane Function

  25. Types of Transport Proteins • Channel proteins are embedded in the cell membrane & have a pore for materials to cross • Carrier proteins can change shape to move material from one side of the membrane to the other

  26. Facilitated Diffusion Molecules will randomly move through the pores in Channel Proteins.

  27. Facilitated Diffusion • Some Carrier proteins do not extend through the membrane. • They bond and drag molecules through the lipid bilayer and release them on the opposite side.

  28. Carrier Proteins • Other carrier proteins change shape to move materials across the cell membrane

  29. Active Transport • Requires energy or ATP • Moves materials from LOW to HIGH concentration • AGAINST concentration gradient

  30. Active transport • Examples: Pumping Na+ (sodium ions) out and K+ (potassium ions) in against strong concentration gradients. • Called Na+-K+ Pump

  31. Sodium-Potassium Pump 3 Na+ pumped in for every 2 K+ pumped out; creates a membrane potential

  32. Moving the “Big Stuff” Exocytosis- moving things out. Molecules are moved out of the cell by vesicles that fuse with the plasma membrane. This is how many hormones are secreted and how nerve cells communicate with one another.

  33. Exocytosis Exocytic vesicle immediately after fusion with plasma membrane.

  34. Moving the “Big Stuff” Large molecules move materials into the cell by one of three forms of endocytosis.

  35. Pinocytosis Most common form of endocytosis. Takes in dissolved molecules as a vesicle.

  36. Pinocytosis • Cell forms an invagination • Materials dissolve in water to be brought into cell • Called “Cell Drinking”

  37. Example of Pinocytosis mature transport vesicle pinocytic vesicles forming Transport across a capillary cell (blue).

  38. Receptor-Mediated Endocytosis Some integral proteins have receptors on their surface to recognize & take in hormones, cholesterol, etc.

  39. Receptor-Mediated Endocytosis

  40. Endocytosis – Phagocytosis Used to engulf large particles such as food, bacteria, etc. into vesicles Called “Cell Eating”

  41. Phagocytosis About to Occur

  42. Phagocytosis - Capture of a Yeast Cell (yellow) by Membrane Extensions of an Immune System Cell (blue)

  43. ExocytosisThe opposite of endocytosis is exocytosis. Large molecules that are manufactured in the cell are released through the cell membrane. Inside Cell Cell environment

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