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Schematic representation of pJD67 construct tagging snrk2.2(are10) and snrk2.2(are16) alleles in Chlamydomonas reinhardtii. Constructed by digesting plasmid pArg7.8 and cloning into pBluescript KS+. Primer sequences used for verification shown.
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Figure S5 Figure S3 - Schematic representation of the pJD67 construct which tags the snrk2.2(are10) and snrk2.2(are16) alleles. pJD67 was constructed by digesting plasmid pArg7.8 (Debuchy et al. 1989) with HindIII and NruI. The resultant 8.3 kb fragment was cloned into the HindIII and SmaI sites of pBluescript KS+ (Stratagene, La Jolla, CA). The positions of the pKS 1 (5’-tcgccgaaaatgacccagagc-3’) and ARG7 lower (5’-cgtacaaggcccatgcgtgagtc-3’) primer sequences that were used to verify the presence of the insert in snrk2.2(are10) genomic DNA, are shown. Literature cited in Figures S3, S4 & S5. Davies, J., F. Yildiz and A. R. Grossman, 1996 Sac1, a putative regulator that is critical of survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO J 15: 2150-2159. Davies, J. P., F. Yildiz and A. R. Grossman, 1994 Mutants of Chlamydomonasreinhardtii with aberrant responses to sulfur deprivation. The Plant Cell 6: 53-63. Debuchy, R., S. Purton and J.-D. Rochaix, 1989 The argininosuccinate lyase gene of Chiamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J 8: 2803-2809.
