1 / 16

Stimulation of In Vivo Angiogenesis Through Dual Growth Factor Loaded Hyaluronic Acid Hydrogels

Stimulation of In Vivo Angiogenesis Through Dual Growth Factor Loaded Hyaluronic Acid Hydrogels. Celeste Riley Mentor: Dr. Robert Peattie Department of Chemical Engineering. Importance. Engineered tissues Organ transplants Ischemic Pathologies (Diabetes). Components of the Project.

lucio
Download Presentation

Stimulation of In Vivo Angiogenesis Through Dual Growth Factor Loaded Hyaluronic Acid Hydrogels

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Stimulation of In Vivo Angiogenesis Through Dual Growth Factor Loaded Hyaluronic Acid Hydrogels Celeste Riley Mentor: Dr. Robert Peattie Department of Chemical Engineering

  2. Importance • Engineered tissues • Organ transplants • Ischemic Pathologies (Diabetes)

  3. Components of the Project • Preparation of Hydrogels • Surgery on living mouse models • Count formed blood vessels and analyze data

  4. Hydrogel Preparation What is a hydrogel? • Natural or Synthetic • Biocompatible • Crosslinked to form a gel-like substance • Dehydration

  5. Hydrogel Preparation What is Hyaluronic Acid? • Glycosaminoglycan • Complexes H20 molecules • Chief component of extracellular matrix • Contributes to cell proliferation and migration Chemical structure of hyaluronic acid http://www.hyalogic.com/aboutha.htm

  6. Addition of Growth Factors What are growth factors? • Signaling proteins • Bind receptors of target cells • Promote differentiation and maturation Diagram of Growth Factor Binding http://www.grahamstudios.net/medcon.html

  7. Addition of Growth Factors Which Growth Factors? • Vascular Endothelial Growth Factor (VEGF) • Angiopoietin 1 (Ang-1) Model of VEGF http://kerrins.net/ud/proteins.html

  8. Make hydrogel from hyaluronic acid Impregnate hydrogel with growth factors Ang-1 and VEGF Dehydrate and implant hydrogel into mouse ear Determine if implant, loaded with growth factors, stimulates angiogenesis Goal of Project GFs +

  9. Project Setup Conditions to consider • Spontaneous Growth • Original Vessels • Individual combinations: control cases (chart)

  10. Treatment Cases • 6 animals per treatment group

  11. Determining Blood Vessel Growth • Thin-section both surgical and contralateral ear • Count blood vessels under 400X magnification Contralateral Ear

  12. Data Analysis What do we want to measure? Define a statistical index to account for • effect of sham surgery • number of vessels present before surgery How do we measure it? Additional vessels due only to the particular surgical treatment

  13. Neovascularization Index NI = (treatment-CL) – (sham-CL) mean CL • (treatment-CL) = average # of additional blood vessels in the implanted ear of treatment group • (sham-CL) = average # of additional vessels due to the surgical procedure itself • mean CL = average vessel count in all contralateral ears: effectiveness measure

  14. Results

  15. Conclusion • Currently, data collection is still ongoing • When calculated, compare NI of experimental case to NIs of control cases • At this point, no definite conclusion can be drawn about the effect of Ang-1 and VEGF delivered through HA hydrogel on angiogenesis

  16. Acknowledgments Dr. Robert Peattie Dr. Kevin Ahern Howard Hughes Medical Institute Co-researcher Peter Fuegy

More Related