1 / 11

C HARACTERIZATION OF S OLID L IPID N ANOPARTICLES

C HARACTERIZATION OF S OLID L IPID N ANOPARTICLES. Cécile Allais # , F. Artzner # , T. Narayanan § , T. Gulik-Krzywicki ‡ , G. Keller # and M. Ollivon # # Equipe de Physico-Chimie des Systèmes Polyphasés, UMR CNRS 8612, Faculté de Pharmacie,

luella
Download Presentation

C HARACTERIZATION OF S OLID L IPID N ANOPARTICLES

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. CHARACTERIZATIONOFSOLIDLIPIDNANOPARTICLES Cécile Allais#, F. Artzner#, T. Narayanan§, T. Gulik-Krzywicki‡, G. Keller# and M. Ollivon# # Equipe de Physico-Chimie des Systèmes Polyphasés, UMR CNRS 8612, Faculté de Pharmacie, 5 Rue Jean-Baptiste Clément 92296 Chatenay-Malabry Cedex, France § E.S.R.F., Grenoble, France ‡Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France cecile.allais@cep.u-psud.fr

  2. Pharmaceutical Point of View: VECTORISATION ofPOORLY WATER-SOLUBLE DRUGS Active compounds:  Hydrophobicity  Crystallinity Characteristics : Nanometric Size: ~100nm  Lipidic Matrix: non toxic  Solid Matrix: slow release System : -model of hydrophobic molecule = CHOLESTEROL - matrix lipid = COCOA BUTTER : mixture of triglycerides

  3. SOLID LIPID NANOPARTICLES 100< d < 300 nm Low concentration of lipids: < 1% (w/w) ? Physical Characteristics: - SHAPE ? - HOMOGENEITY ? - POLYMORPHISM ? Techniques Optic Microscopy X-ray Diffraction Electronic Microcopy

  4. OPTIC MICROSCOPY IN DARK FIELD hn Light scattering Cocoa Butter/Cholesterol Nanoparticles

  5. hn OPTIC MICROSCOPY IN DARK FIELD Nanoparticles Stocked 1 Month at 4°C Nanoparticles just After Preparation at Room Temperature Spherical Particle Non Spherical Particle

  6. 100 nm 100 nm 100 nm 100 nm ELECTRONIC MICROSCOPY (T. Gulik-Krzywicki) Presence of Flat and Hemispherical Sides

  7. Cocoa Butter Polymorphism in Bulk: I < II < III < IV < V < VI[Loisel et al., 1998] 2L 3L After making at 10°C After quenching at 8°C 44.2 Å 48.2 Å 48.6 Å 54.3 Å Intensity (a.u.) I + II/III II/III + IV Intensity (a.u.) 27.15 Å 16.2 Å 16.1 Å 14.7 Å q (Å-1) q (Å-1) COCOA BUTTER POLYMORPHISM IN NANOPARTICLES (SAXS on ID2 Beamline, E.S.R.F., T. Narayanan) Cocoa Butter Nanoparticles X-ray patterns at10°C [Lipids]eau<1% (w/w)

  8. 47.8 Å 44.2 Å 47.8 Å 44 Å II/III + IV + CHOLESTEROL II/III + IV + CHOLESTEROL Intensity (a.u.) Intensity (a.u.) 34.5 Å 34.5 Å 16.1 Å 16.1 Å 17.1 Å 17.1 Å 14.5 Å 14.7 Å q (Å-1) q (Å-1) Making at 10°C Tempering at 30°C POLYMORPHISM CONTROL ? (SAXS on ID2 Beamline, E.R.S.F., T. Narayanan) CB/Cholesterol 50/50 (w/w) Nanoparticles X-ray patterns at10°C [Lipids]eau<1% (w/w)

  9. -40°C -38°C -36°C -34°C -32°C -30°C TmeltingNanoparticles< Tmelting Bulk Only 2L structures: -28°C -26°C -24°C -22°C -20°C -18°C I < II < III < IV < V < VI -16°C 2L 3L -14°C -12°C -10°C q (Å-1) POLYMORPHISM CONTROL ? (SAXS on ID2 Beamline, E.S.R.F., T. Narayanan) CB/Cholesterol 50/50 (w/w) Nanoparticles

  10. Dark-Field Microscopy Electronic Microscopy X-ray Diffraction Particle Model CONCLUSION  MultiTechniques Approach: -size -shape  Non Homogeneous Particles: microsegregation Polymorphism Study: low concentration: 1% (w/w) 2L

  11. Vacuum tube containing detector Mobile sample carrier with a capillary tube Incident X-ray beam X-RAY DIFFRACTION (T. Narayanan, E.S.R.F., Grenoble) ID 2 Beamline

More Related