An Engineered Liver Glycogen Phosphorylase with AMP Allosteric Activation*

Sep 10, 2014

90 likes | 170 Views

An Engineered Liver Glycogen Phosphorylase with AMP Allosteric Activation*.

Share Presentation

Embed Code

Link

california
texas 75235
gifford laboratories
diabetes research
amp allosteric activation
engineered liver glycogen phosphorylase

lynne

Download Presentation

An Engineered Liver Glycogen Phosphorylase with AMP Allosteric Activation*

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

An Engineered Liver Glycogen Phosphorylase with AMP Allosteric Activation* Ward S . Coats$, Michelle F. Brownersli, Robert J. Flettericks, and Christopher B. NewgardSIIFrom the $Department of Biochemistry and Center for Diabetes Research, Gifford Laboratories, University of TexasSouthwestern Medical Center, Dallas, Texas 75235 and the $Department of Biochemistry and Biophysics, university ofCalifornia, San Francisco, California 94143-0448

Glycogen Metabolism

Glycogen Metabolism. Purpose: Glycogen is a branched polymer of glucose; it is the stored form of G. The many branches each have a C#4 end at which GP and GS can act for rapid response. Glycogen is stored after a meal for release:

1.57k views • 47 slides

Factors that affect Protein Activity and Protein Mechanism

Factors that affect Protein Activity and Protein Mechanism. Chapter 6 (Page 212-218, 225-231). 1. Factors that affect Enzyme Activity. Inhibitors Proteolytic activation Protein modifications Subunit cooperativity and modulators pH. 1I. Proteolytic Activation.

1.58k views • 53 slides

Glycogen Phosphorylase

Carlos Mendoza. Lee Sutton. Glycogen Phosphorylase. Kristen Harris. Glycogen Phosphorylase is an enzyme found in glycogen granules in the cytosol

1.13k views • 24 slides

Glycogen Storage Diseases

Glycogen Storage Diseases. Type I. Name? . VON GIERKE’S DISEASE. Severe fasting hypoglycemia, way increased liver glycogen, increased blood lactate, hepatomegaly Glucose-6-Phosphatase (If you don’t dephosphorylate glucose, cells cant release it!). Symptoms? Enzyme Def?. Type V. Name? .

1.15k views • 10 slides

The Regulation of Eukaryotic Gene Expression

PEPCK. This is an acronym for an enzymePhosphoEnol Pyruvate CarboxyKinaseThis enzyme is ONLY regulated by gene expression!No allosteric activators, covalent modification etcNo activation by cAMP, inhibition by insulin etc. PEPCK. The enzyme is expressed in liver, kidney, adipose tissue and to a

1.14k views • 59 slides

Overview: Carbohydrates

Overview: Carbohydrates. Importance of carb storage Muscle & liver How many carbs are right for you? What to eat before, during and after exercise to enhance performance. Glycogen. The way the body stores glucose Limited storage Stored in muscle & liver

332 views • 21 slides

PHOSPHOROLYSIS OF a (1-4)LINKAGES UP TO 4 GLUCOSE RESIDUES FROM AN a (1-6)BRANCH POINT

GLYCOGEN BREAKDOWN. PHOSPHORYLASE. (GLUCOSE)n + Pi (GLUCOSE)n-1 + G-1-P. PHOSPHOROLYSIS OF a (1-4)LINKAGES UP TO 4 GLUCOSE RESIDUES FROM AN a (1-6)BRANCH POINT. GLYCOGEN SYNTHESIS. HEXOKINASE. GLUCOSE + ATP GLUCOSE-6-PHOSPHATE + ADP. PHOSPHOGLUCOMUTASE.

557 views • 17 slides

mitochondrion

CARBOHYDRATES. Monosaccharides Found in the cytosol and in organelles Use for energy production and in biosynthesis. Simple polysaccharides Glycogen and starch polymers of glucose Glycogen in granules in cytoplasm in muscle and liver cells. Rough endo . reticulum. ribosome.

121 views • 1 slides

LIVER

LIVER. What is the liver, and what does it do? How a liver become fatty Liver Stones How the Body is affected How alcohol affects the body Life Style. What is the liver, and what does it do ?.

2.71k views • 23 slides

Liver transplantation

Liver transplantation. Is the replacement of a diseased liver with a healthy liver allograft. Used technique is orthotopic transplantation, in which the native liver is removed and replaced by the donor organ in the same anatomic location as the original liver.

1.11k views • 26 slides

The Ras – MAP kinase pathway

The Ras – MAP kinase pathway. I. The big family of Receptor Tyrosine Kinases. Receptor domain architecture and receptor activation by EGF (TGF a ). unusual mode of allosteric activation, formation of an asymmetric dimer, followed by trans-phosphorylation of the C-tails. II. II.

1.25k views • 27 slides

Blood Glucose

Anaerobic Glycolysis. Sarcolemma. Glycogen. Phosphorylase. Hexosephosphate Isomerase. (+) insulin. H K. Glucose. Blood Glucose. G-6-P. F-6-P. (-)ATP. PFK. ATP ADP. ATP ADP. F-1,6-P. ATP ADP. Aldolase. G3P DH. (2) 3PG. (2) 1,3 DPG. (2) G-3-P. DHAP. PG Kinase.

270 views • 4 slides

GLYCOGEN BREAKDOWN AND SYNTHESIS ARE RECIPROCALLY REGULATED

GLYCOGEN BREAKDOWN AND SYNTHESIS ARE RECIPROCALLY REGULATED. Glycogen phosphorylase muscle liver Allosteric inactive “b” active “a” Regulation AMP: T to R glucose: R to T Covalent epinephrine glucagon (PKA) Regulation through PKA, Ca epinephrine (beta, alpha adr.)

382 views • 22 slides

Allosteric enzymes

Allosteric enzymes. Allosteric enzymes tend to be multi-sub unit proteins The reversible binding of an allosteric modulator (here a positive modulator M) affects the substrate binding site. Kinetics. Models. Cooperation. v o. (+). [S]. (+). v o. (+). [S]. v o. (-). (-). [S].

2.89k views • 27 slides

GLYCOGEN METABOLISM

GLYCOGEN METABOLISM. Glycogen Structure. Most of the glucose residues in glycogen are linked by a -1,4-glycosidic bonds. Branches at about every tenth residue are created by a -1,6-glycosidic bonds. Glycogen is an important fuel reserve for several reasons.

977 views • 49 slides

Hepatic Glycogenolysis

Hepatic Glycogenolysis. regulated by hypoglycemic signals. phosphorylase b. Contrast: Skeletal Muscle Glycogen Utilization. anaerobic glycolysis. Cori cycle. hepatic gluconeogenesis. Muscle lacks G6 PTPase Glycogen conversion to lactate is not regulated by

760 views • 27 slides

Intracellular distribution of glycogen synthase and glycogen in primary cultured rat hepatocytes

Intracellular distribution of glycogen synthase and glycogen in primary cultured rat hepatocytes. Mar GARCIA-ROCHA*, Angela ROCA*, Nuria DE LA IGLESIA*, Otto BABA., Josep M. FERNANDEZ-NOVELL*,Juan C. FERRER* and Joan J. GUINOVART*1.

192 views • 8 slides

Lecture 8 - Glycogen Metabolism

Lecture 8 - Glycogen Metabolism. Chem 454: Regulatory Mechanisms in Biochemistry University of Wisconsin-Eau Claire. Introduction. Glycogen A storage form of glucose. Introduction. Glycogen is stored primarily in the liver and skeletal muscles.

916 views • 41 slides

Glycogen

Glycogen. Glycogen. Liver ~ 4 % 72 g Muscle ~ 1 % 245 g Extracellular glucose 0,1 % 10 g Adult - man 70 kg His liver 1,8 kg His muscle mass 35 kg Blood volume 10 l. Glycogen. Muscle: Glucose substrate for muscle glycolysis Liver: Glucose store, Export of hexose Blood sugar

814 views • 31 slides

PRINCIPLES OF METABOLIC REGULATION: GLUCOSE AND GLYCOGEN

PRINCIPLES OF METABOLIC REGULATION: GLUCOSE AND GLYCOGEN. Lehninger Ch. 15 BIO 322 Recitation 2 / Spring 2013. Glycogen stored - %10 weight of the liver 0.4 M in a hepatocyte if dissolved as glucose – impaired osmotic properties (As glycogen 0.01 μ M) Liver glycogen can be depleted 12-24 hrs

595 views • 26 slides

More Related