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ncRNA

ncRNA. Jeremy Logue. The Expanding Repertoire of RNA. Adapted from Soares and Valcarcel (2006) EMBO. The Expanding World of Non-coding RNAs. Adapted from Huttenhofer, A et al. (2005) Trends in Genetics . HAR1-associated transcripts in genomic context.

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ncRNA

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  1. ncRNA Jeremy Logue

  2. The Expanding Repertoire of RNA Adapted from Soares and Valcarcel (2006) EMBO

  3. The Expanding World of Non-coding RNAs Adapted from Huttenhofer, A et al. (2005) Trends in Genetics

  4. HAR1-associated transcripts in genomic context Of the 49 HARS identified (Human Accelerated Regions), 96% are in non-coding regions.

  5. Predicted RNA secondary structure for HAR1F

  6. Expression of HAR1F and HAR1R in the developing neocortex Cajal-Retzius cells establish early neuronal circuitry in the developing brain

  7. Expression of HAR1F in other parts of the developing brain

  8. What does this all mean? Fill in the blank…

  9. X - Inactivation

  10. Dosage Compensation Adapted from Straub and Becker (2007) Nature Reviews Genetics

  11. Imprinted Versus Random X-inactivation Adapted from Avnir and Heard (2001) Nature Reviews Genetics

  12. The X-inactivation Center Adapted from Avnir and Heard (2001) Nature Reviews Genetics

  13. Xist Transcription in Embryonic Stem Cells 48 hrs. Differentiation Adapted from Avnir and Heard (2001) Nature Reviews Genetics

  14. Potential Roles for Xist Antisense Transcription Adapted from Avnir and Heard (2001) Nature Reviews Genetics

  15. What does a silenced X look like? Adapted from Chow, JC et al. (2005) Annu. Rev. Genomic Hum. Genet.

  16. Co-localization and 3D - Microscopy

  17. How confident are we that these co-localize?

  18. Problems Associated with Co-localization Resolution = λ/(2NA) z-axis problem Figure 1G from Chaumeil, J. et al. (2007) Gen Dev Calculating a cross-correlation can also aid in determining co-localization

  19. 3D - Microscopy

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