1 / 6

Recombinant DNA Bacterial Transformation Student Instructions Mid-Log Culture Prep

Recombinant DNA Bacterial Transformation Student Instructions Mid-Log Culture Prep. Recombinant Transformation Day 1 Overnight Culture Plate. Using the extra “LB” agar plate, streak the MM 294 E.coli culture on the plate and incubate 18-24 hours. Recombinant Transformation Day 2

malcolm-gamble
Download Presentation

Recombinant DNA Bacterial Transformation Student Instructions Mid-Log Culture Prep

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Recombinant DNA Bacterial Transformation Student Instructions Mid-Log Culture Prep

  2. Recombinant Transformation Day 1 Overnight Culture Plate Using the extra “LB” agar plate, streak the MM 294 E.coli culture on the plate and incubate 18-24 hours.

  3. Recombinant Transformation Day 2 Mid-Log Culture Label a sterile 50 ml conical tube with your name and date.

  4. Recombinant Transformation Mid-Log Culture Use a sterile pipette to transfer one pipette full (5 ml) of sterile LB broth into the conical tube.

  5. Recombinant Transformation Mid-Log Culture Locate a well-formed colony from your plate of bacteria grown the day before. Use a sterile inoculating loop, bent paper clip, or toothpick to scrape up the colony and transfer it to the LB broth in the conical tube. Your instructor may have started the culture several hours before class.

  6. Recombinant Transformation Mid-Log Culture Incubate the tube for 3-4 in a shaking water bath or use an orbital shaker in an incubator. Alternatively: Incubate 4 hours in the incubator with occasional shaking by hand. For best results, shake the tube a couple of times an hour when possible. After the 4 hour incubation period, immediately proceed to the “Making Competent Cells” protocol.

More Related