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Forensic Entomology:

Forensic Entomology: Identification of fly species for estimation of Post Mortem Interval (PMI) in UMMC. ParaSEAD Laboratory Department of Parasitology , Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia . Introduction:

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Forensic Entomology:

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  1. Forensic Entomology: Identification of fly species for estimation of Post Mortem Interval (PMI) in UMMC ParaSEAD Laboratory Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

  2. Introduction: • Entomology can be defined is short as a studies on insects. • Therefore, Forensic Entomology is defined as

  3. The use of the insects to aid legal investigations. • It is commonly broken down into three general areas: • Medicolegal • Urban and • Stored product

  4. Medicolegal focuses on the criminal component of the legal system and deals with feeding insects that typically infest human remains (commonly use to determine time of death). • Urban aspect deals with the insects that affect man and his immediate environment (relates to interpretation on damage caused). • Stored product insects are commonly found in foodstuffs (relates to food contamination).

  5. Maggot

  6. What is maggot? It is one of the stages found in the life-cycle of fly.

  7. Life-cycle of fly (23 hours) Eggs 1st, 2nd, 3rd larvae (130 hours) (143 hours) Adult fly pupae

  8. Corpse

  9. Maggots Corpse with maggots Maggots

  10. Corpse in different situations

  11. Objective • To identify fly species for estimation of PMI (Post-mortem inverval)

  12. Materials:

  13. Methods: Alive sample Fixed sample Transfer into containers with meat Examine for specific characteristics Allow to develop until pupa stage Allow to develop until L3 stage Transfer to develop into adult stage Slide preparation Sacrifice Determine species Mounted Determine species

  14. Mounted adult fly

  15. Slide of maggot

  16. Anterior Spiracle Identification of the specific characteristics of the maggot

  17. 1. Measuring the length of larvae

  18. 2. Characterising the anterior spiracle

  19. 3. Characterising the posterior spiracle

  20. Based on the characteristic of posterior spiracle, the maggot can be grouped as follows:

  21. Results: Species of maggot identified in Para:SEAD Laboratory (2003-2011)

  22. Conclusion: • The number of fly species identified is 9 • The most common species is Chrysomyamegachepala(52%) followed byC.rufifacies(21%). • The least common species are Chrysomyabezzianaand Hermetriaillucens

  23. References: A Guide To Medical Entomology Author: M.W service Insect and Forensic Entomology Author : Ian Dadour (Entomologist , University of WA & David Cook (Entomologist, Agriculture WA ) Manual of Medical Entomology, Author: Deane P. Furman and Elmar P. Catts Myiasis In Man And Animals In Old Word, Author: F. Zumpt DPDx- CDC Laboratory Identification of Parasites of Public Health concern

  24. Thank You

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