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SUPPLEMENTARY FIGURES • Figure S1. Cell viability using trypan blue exclusion assay. MDCK cells were treated with COM (500 μg crystals/ml medium),50 µM nystatin, 50 µM cytochalasin D, or 50 µM CPZ for 15 min. The cells without any treatment served as controls. The data are presented as mean ± SD. • Figure S2.Number of adhered and/or internalized crystals after inhibitor treatment. MDCK cells were pretreated with 50 µM nystatin (A) or CPZ (B) for 15 min before incubation with COM crystals for 2 h. Unbound crystals were removed by extensive washes. The adhered and/or internalized crystals were counted and are reported as mean ± SD.
Supplementary Figure S1 Cell viability (%) (500 μg/ml) (50 μM) (50 μM) (50 μM)