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혈액검사

혈액검사. 면역표현형 검사. 골수검사. 세포유전학. 특수혈액. 분자유전학. 일반혈액. 혈액응고. 일반혈액 검사항목. CBC : RBC Hemoglobin(Hb) Hematocrit(Hct) RBC index : MCV, MCH, MCHC WBC Platelet Reticulocyte Eosinophil counts ESR Blood film : WBC differentil PB morphology Malaria parasites. 특수혈액검사 혈액응고검사.

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혈액검사

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  1. 혈액검사 면역표현형 검사 골수검사 세포유전학 특수혈액 분자유전학 일반혈액 혈액응고

  2. 일반혈액검사항목 CBC : RBC Hemoglobin(Hb) Hematocrit(Hct) RBC index : MCV, MCH, MCHC WBC Platelet Reticulocyte Eosinophil counts ESR Blood film : WBC differentil PB morphology Malaria parasites

  3. 특수혈액검사 혈액응고검사 Bleeding time Prothrombin time aPTT Thrombin test Fibrinogen Factor assay FDP test Paracoagulation test Antithrombin III Lupus anticoagulant Clot retraction time Clot lysis time Platelet function test Osmotic fragility Autohemolysis test Ham's test Sucrose hemolysis test Fetal hemoglobin LE cell test LAP score NBT test BM aspiration & biopsy Chromosome study BM cell special stain Molecular study Immunophenotyping

  4. 혈액검사용 검체채취 • Equipment : Lancet, glass capillary • Plastic syringe with needle • Vacuum system • Skin puncture : Capillary blood • 3rd/4th finger : Blood smear • Ear lobe : Bleeding time • Venipuncture : antecubital vein • 23-gauge ..... 3- 5ml • 21-gauge ..... 5-10ml • 20-gauge ..... 10-15ml • 18-gauge ..... 30-50ml

  5. 신생아와 소아의 혈액채취 • Capillary blood : heal or first toe • Cord blood • External jugular vein • Femoral vein

  6. 항응고제 및 관련 혈액검사 • EDTA .................................일반혈액검사 • 백혈병표지검사 • 분자유전학검사 • Sodium citrate ...................혈액응고검사, ESR • Heparin • Sodium Heparin ....... 특수혈액검사 • Preservative-free Heparin ....염색체검사 • 세포배양 • Defibrillation

  7. 혈액검체의 보관 • 2시간 이상 검사지연 : 냉장보관 • 2시간내 검사 : ESR, PT • 즉시 검체분리 : Cold agglutinin (혈장 실온보관) • 혈액검체 저장시 변화 • 물리적 변화 : autoagglutination, • plasma viscosity 증가 • 적혈구 : swelling, crenation, sphering • 중성구 : vacuolation, nuclear swelling, • loss of granules • 림프구,단구: "clover-leaf" appearance • with vacuolation

  8. 혈장과 혈청의 차이점 전혈 + 항응고제 혈장(plasma) 전혈 혈청(serum) Blood clot : RBC +WBC+ Platelet +Factor I, II, V, VIII

  9. Different Layers of Venous Blood Plasma Total volume blood Platelets WBCs Buffy coat Reticulocytes RBCs

  10. 혈액성분별 검사내용 Plasma 혈액응고검사 특수혈액검사 말라리아검사 악성세포검출 Buffy coat smear

  11. 혈액은행 수혈혈액 • Red blood cells (RBC) 200-250ml • Fresh frozen plasma (FFP) 160-200ml • Whole blood (WB) 400-450ml • Platelet concentrate (PC) 40ml • Platelet-rich plasma (PRP) 200ml • Washed RBC (WRBC) 250ml • Cryoprecipitate (CRYO) 30ml Anticoagulant : CPDA-1

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