1 / 4

Mock

Figure 1. shGld2+ Gld2-HA. a. b. Mock. shGld2. Gld2-HA. Gld2-HA. Gld2. Tubulin. Tubulin. c. d. shGld2. shGld2. Mock. shGld2. Mock. p53. A 200. Actin. A 0. e. shGld2. f. Mock. shGld2. 8. 7. Mock. 6. 5. Oxygen Consumption (AU). shCPEB. 4. 10. Mock. 8. 3.

moke
Download Presentation

Mock

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Figure 1. shGld2+ Gld2-HA a b Mock shGld2 Gld2-HA Gld2-HA Gld2 Tubulin Tubulin c d shGld2 shGld2 Mock shGld2 Mock p53 A200 Actin A0 e shGld2 f Mock shGld2 8 7 Mock 6 5 Oxygen Consumption (AU) shCPEB 4 10 Mock 8 3 shGld2 Population Doublings 6 2 4 1 2 0 0 0 75 15 30 45 60 90 0 3 6 9 Time (min) Days

  2. Figure 2. a b TK-Rluc CPEB-Full-3’-UTR 500 CPEB-Full-5’-Half TK-Rluc ΔB 400 TK-Rluc Relative % to mock (normalized to actin) ΔC TK-Rluc 300 ΔD TK-Rluc 200 ΔE TK-Rluc 100 Fluc control TK-Fluc 0 CPEB Intron CPEB Exon d c 400 CPEB-Full-3’-UTR CPEB-Full-5’-Half 200 300 150 Relative % (Rluc/Fluc) Relative % (Rluc/Fluc) 200 100 100 50 0 0 Full Mock shGld2 ΔB ΔC ΔD ΔE

  3. Figure 3. a d TK-Rluc CPEB-Full-3’-UTR 12 Relative mir122 exp. (normalized to actin) 10 TK-Rluc Δ122-1 8 TK-Rluc Δ122-2 6 TK-Rluc Both 4 2 Fluc control TK-Fluc 0 shGld2 WT Anta-122 Anta-Scr 250 Mock e 200 Relative % (Rluc/Fluc) 150 35S-p53 100 50 Both Δ122-1 Δ122-2 0 WCE CPEB-Full f b 200 150 % p53 protein 100 50 0 Mock Anta-122 Anta-Scr c shTETR shCPEB g 400 Anta-Scr Anta-Scr Anta-122 Anta-122 Mock Mock 300 Relative % (Rluc/Fluc) 35S-p53 200 100 0 Mock WCE Anta-122 Anta-Scr

  4. Figure 4. shMPAP shGld4 Mock d a shMPAP Mock shGld4 A200 p53 A0 Actin e Intron Exon b 200 shTETR shCPEB p53 expression (relative to actin) 150 100 50 IP Flag-Gld4 IP Flag-Gld4 20% input 20% input 0 IgG IgG Mock Mock shMPAP shMPAP shGld4 shGld4 p53 GAPDH f Mono-adenylated miR122 Gld2 miR122-RISC A A A 5% input IgG IP CPEB c CPEB up CPEB down A A CPEB CPEB Gld4-HA p53 down p53 up Gld4 Mcl1-HA CPEB p53 p53 AAAAAAAAAAA A

More Related