1 / 2

Hemocytometer ( 血球計數器 ) 介紹

Hemocytometer ( 血球計數器 ) 介紹. 由此滴加樣本. http://www.ruf.rice.edu/~bioslabs/methods/microscopy/cellcounting.html. 細胞濃度計算. Volume ( 體積 ) = 長 x 寬 x 深度 = 1 mm x 1 mm x 0.1 mm = 0.1 mm 3 = 1 x 10 -4 cm 3 (mL) 若該體積有 n 個細胞，則細胞濃度為 = n 個 / (1 x 10 -4 mL) = n x 10 4 個 /mL ( 若有稀釋需再乘上稀釋倍率 )

more
Download Presentation

Hemocytometer ( 血球計數器 ) 介紹

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Hemocytometer (血球計數器)介紹 由此滴加樣本 http://www.ruf.rice.edu/~bioslabs/methods/microscopy/cellcounting.html

  2. 細胞濃度計算 • Volume (體積) • =長 x 寬 x 深度 • = 1 mm x 1 mm x 0.1 mm • = 0.1 mm3 • = 1 x 10-4 cm3 (mL) • 若該體積有n個細胞，則細胞濃度為 • = n個 / (1 x 10-4 mL) • = n x 104 個/mL • (若有稀釋需再乘上稀釋倍率) • 常使用計數ABCD四區域有n個細胞，則細胞濃度為 • = n / 4 x 104 （個/mL） • 細胞計數時，細胞濃度不要太稀，太稀準確度會比較低，最好在100～300 x 104 個/mL A B C D 1 mm 1 mm Hemocytometer (血球計數器)

More Related