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MITOSIS

MITOSIS. Text and image sources are included using the notes function of this file. Newts are cute. Video. And Kangaroo Rats keep you hopping!. Video. The Cell Cycle. G2. G1. M. START. S. DNA Replication Factories. HeLa cell. Yeast cells. Centriolar replication in S.

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MITOSIS

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  1. MITOSIS Text and image sources are included using the notes function of this file

  2. Newts are cute Video

  3. And Kangaroo Rats keep you hopping! Video

  4. The Cell Cycle G2 G1 M START S

  5. DNA Replication Factories HeLa cell Yeast cells

  6. Centriolar replication in S

  7. Mitotic Entry - PreProphase Nuclear envelope breakdown Endomembrane system vesiculation Cytoskeletal remodeling MPF

  8. Monastrol inhibits Eg5 and MTOC resolution + + Both in vivo and in vitro

  9. Chromosomes condense in an orderly manner

  10. Structural Maintenance of Chromosomes involves both cohesins (SMC1+3) and condensins (SMC2+4)

  11. Prophase

  12. Three ways to make spindles (in vitro) +beads +Sperm Nuclei and Ca++ +MTOCs

  13. Spindle assembly in vitro 2 Videos

  14. Dynein focuses the poles

  15. Kinetochore assembly Adenomatous Polyposis Coli protein is a tumor supressor involved in cell adhesion, migration, and division (Calcinosis, Raynaud's, Esophageal dysmotility, Sclerodactyly, Telangiectasia)

  16. CENP-E Antibody depleted; note orphaned chromosomes 20um 20um 10um 10um

  17. Fast moves Video

  18. Aurora Kinases Aurora A at spindle poles; Aurora B at centromeres

  19. Alignment of the kinetochore and pole-to-pole microtubules Video

  20. Metaphase

  21. Tug of war CENP-B::GFP Video CENPB

  22. Microtubule Treadmilling in the Spindle Video

  23. Metaphase Checkpoint Mad2 signals unoccupied kinetochores MAD2 Video APC Securin Separase Cohesin Sister Chromatid Separation

  24. Anaphase Anaphase A Anaphase B

  25. In vitro anaphase Video

  26. Cytokinesis Video

  27. Cytochalasin D Video

  28. GFP-Actin Video

  29. Kinases rule! Plk1 -> Ect2 (GEF) -> RhoA Prometaphase Metaphase Anaphase A Anaphase B Aurora B Kinase RNAi of Plk1 = cytokinetic failure

  30. Rho activation at the cleavage furrow GFP::Rhotekin Rho Binding Protein localizes to activated Rho Video

  31. Myosin activation at the furrow DNAActive Myosin

  32. The Midbody CENP-E Cep55 coordinates mt bundling and SNARE-mediated cell detachment CENP-E, Aurora B, and other key mitotic molecules and motors are trapped in the midbody at the end of mitosis.

  33. Lamin associated protein alpha::FP Video H2B

  34. Golgi and endomembrane reassembly Video GFP-GalTase

  35. Fitting it all together Video

  36. Plant Mitoses End Differently Video With the formation of a phragmoplast barrier

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