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LEUKOCYTE TRAFFIC : SELECTINS, CHEMOATTRACTANTS, INTEGRINS AND CAM D. Dunon

LEUKOCYTE TRAFFIC : SELECTINS, CHEMOATTRACTANTS, INTEGRINS AND CAM D. Dunon. Movies: - Timothy A. Springer http://cbr.med.harvard.edu/springer - Eugene Butcher - Ulli von Adrian - Richard Thompson. LEUKOCYTE-ENDOTHELIUM INTERACTION. Stamper and Woodruff.

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LEUKOCYTE TRAFFIC : SELECTINS, CHEMOATTRACTANTS, INTEGRINS AND CAM D. Dunon

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  1. LEUKOCYTE TRAFFIC : SELECTINS, CHEMOATTRACTANTS, INTEGRINS AND CAMD. Dunon Movies: - Timothy A. Springer http://cbr.med.harvard.edu/springer - Eugene Butcher - Ulli von Adrian - Richard Thompson

  2. LEUKOCYTE-ENDOTHELIUM INTERACTION

  3. Stamper and Woodruff

  4. ADHESION MOLECULES

  5. Intravital microscopy. The mouse mesentery preparation. The following in vivo microscopy is of postcapillary venules from a different type of preparation, hamster cheek pouch Timothy A. Springer, Harvard Medical School

  6. Rolling and Arrest of Leukocytes May Be Reconstituted in vitro with Purified Components ICAM-1 + P- Selectin ICAM-1 Drop of liposomes containing purified adhesion molecules P- Selectin Liposomes bind to glass and fuse to form a continuous membrane bilayer

  7. Timothy A. Springer, Harvard Medical School

  8. Timothy A. Springer, Harvard Medical School

  9. Rolling of neutrophils on purified P-selectin X.MOV_P-sel_low_high

  10. Traffic Signals for Neutrophil Localization in the Vasculature G Protein-Coupled Receptor Active Integrin Carbohydrate Ligand Inactive Integrin Chemoattractant Selectin Ig Family Member Rolling Activation Firm Adhesion, Transmigration

  11. Seven- membrane Mucin-like spanner G Protein Molecule Integrin Ig Family Selectin Chemoattractant Member Step 1 Step 2 Step 3 Reconstitution of neutrophil rolling and arrest in vitro THREESTE.MOV

  12. Reconstitution of Rolling, Activation, and Firm Adhesion in Vitro G Protein-Coupled Receptor Inactive Integrin Active Integrin Carbohydrate Ligand Step 2. Addition of chemoattractant to perfusate solution P- Selectin ICAM-1 Step 3. Firm Adhesion Step 1. Rolling

  13. SELECTINS AND THEIR LIGANDS

  14. Carbohydrate ligands of selectins

  15. dV dr = shear (s-1) Hydrodynamics of Blood Wall V Centerline dV dr Shear x viscosity = shear stress (dynes/cm2) Shear stress in postcapillary venules : 1 to 10 dynes/cm2

  16. Rolling above and detachment below a shear threshold SHEARTHR.MOV

  17. Model of shear stress effect on P selectin

  18. Lymphocyte rolling on purified selectins NEUT_SEL.MOV_lymph_E,L,PNAD

  19. Integrins are activated by selectins and chemokines

  20. Chemokine modify cell shape

  21. Chemokines and their Receptors

  22. LOCALIZATION OF TCA-4, A PLN SPECIFIC CC CHEMOKINE

  23. NAIVE T CELL ADHESION TO PLN-HEV REQUIRES TCA-4

  24. INTRACUTANEOUS INJECTION OF TCA-4 LEADS TO SPECIFIC PRESENTATION BY PLN HEV

  25. Chemokine transduction pathway

  26. Les intégrines et leurs ligands

  27. C S S C C C C C S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S Ig-CAMs NH2 NH2 C S S NH2 C C NH2 C NH2 C C C C C C C C C C NH2 C C C C C S S C C C C C C S S C C C C VCAM ICAM-1 ICAM-3 ICAM-2 ICAM-4 ICAM-5 PECAM

  28. Integrin activation by chemokines

  29. Adhesions molecules in homing

  30. ADHESION MOLECULES INVOLVED IN TRANSENDOTHELIAL MIGRATION

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