1 / 8

Exp.6 Determination of total and direct bilirubin

Exp.6 Determination of total and direct bilirubin. Bilirubin : is the yellow breakdown product of normal haemoglobin. Bilirubin is excreted in bile and urine. Direct and Indirect bilirubin :. Clinical significance:. An increase in bilirubin conc. Is called jaundice.

ozzie
Download Presentation

Exp.6 Determination of total and direct bilirubin

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Exp.6Determination of total and direct bilirubin

  2. Bilirubin: is the yellow breakdown product of normal haemoglobin. • Bilirubin is excreted in bile and urine.

  3. Direct and Indirect bilirubin:

  4. Clinical significance: • An increase in bilirubin conc. Is called jaundice. • High level of direct type means that the bile is not being properly excreted. • High levels of indirect one indicate that too much haemoglobin is being damaged.

  5. Elevation of total bilirubin occur due to: • Excessive hemolysis of RBC • Liver diseases e.g. hepatitis &cirrhosis • Obstruction of biliary tract e.g. gall stones

  6. Principle: • Bilirubin + diazotized sulfanilic acid azobilirubin • The intensity of purple color is α to bilirubin conc. In serum • Bilirubinglucuronide +dizorgt.coloreddiazo compound(1min) • Unconjugated accelerates by methanol to form it • Total bilirubin is obtained after standing 5min colored formed measured at540nm

  7. Calculations: • Direct bilirubin(1min): • Abs(T)- Abs(test blank) x2.5 • Abs(bilirubin equivalent std) • Total bilirubin(5min): • Abs(T)- Abs(test blank) x5.0 • Abs(bilirubin equivalent std)

  8. Expected values in serum: • Direct ……………up to 0.5mg/dl • Total………………up to 1.0 mg/dl

More Related