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ELISA Workshop

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ELISA Workshop

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    1. ELISA Workshop Dr. Mariluci T. Bladon Director, Biotechnology Program Middlesex Community College

    2. What is ELISA? Enzyme Linked Immunosorbent Assay Used for detection of infectious diseases, pregnancy, Lyme’s disease and various types of cancer

    3. Detection Signals Enzyme-substrate interaction Fluorescence signal Radioisotope signal

    6. Materials See Handout 96 well plate

    7. 96 Well Plate

    8. Methods See Handout

    12. ELISA Plate Reader

    13. Results and Conclusion Role of positive and negative controls Role of human in error in unexpected results

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