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Pseudomonas Identification

Pseudomonas Identification. Done By : Omniya Tayem Dr: Adbelraouf Elmanama Mohamad Laqan. Structure & Physiology. Pseudomonas. * Gram _ Negative rods. Gram negative rod. Motile with polar flagella *.

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Pseudomonas Identification

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  1. Pseudomonas Identification Done By : Omniya Tayem Dr: Adbelraouf Elmanama Mohamad Laqan

  2. Structure & Physiology Pseudomonas • * Gram _ Negative rods Gram negative rod

  3. Motile with polar flagella *

  4. Motility positive

  5. Obligate aerobe

  6. Oxidase _ positive *

  7. Do not ferment Carbohydrates

  8. Resistant to multiple drugs

  9. Majority of human infections _ P. aeruginosa

  10. Common in the environment . Water . Air .Soil

  11. Very simple nutritional requirements Many organic compound used as C & N sources , but only afew carbohydrates by oxidative metabolism

  12. Some strains appear mucoid due to Polysaccharide Capsule

  13. Some produce diffusible pigments Pyocyanin _ nonfluorescent bluish pigment Pyoverdin_ Fluorescent greenish pigment Pyorubin & Pyomelanin

  14. Fluorescent Pigments White Light source Black Light source

  15. Pseudomonas . aeruginosa

  16. Pseudomonas aeruginosa colonies on Agar

  17. Pseudomonas . aeruginosa

  18. Identification of P. Aeruginosa Usually based on colonial morphology , B- hemolysis , Oxidase positivity , The presence of characteristic pigments & Sweet odor & Growth at 42c

  19. Identification Of Pseudomonas Pigments* Pyocyanin (blue _ green ) Fluorescein (green _yellow_fluorescent ) Pyorubin (red _ brown )

  20. Culures have fruity smell *

  21. Biochemical reactions * .Gelatine hydrolysis .Starch hydrolysis .Carbohydrates use Trehalose (O_F medium) .Flourescent Pigment

  22. Results

  23. P. aeruginosa Gelatin hydrolysis +/ - ( variable result ) Starch hydrolysis + / - ( variable result ) Acid Aerobically from D_ Trehalose ( negative ) Fluorescent pigment ( positive ) Non Fluorescent pigment ( positive )

  24. P .fluorescens Gelatin hydrolysis ( positive ) Starch hydrolysis ( negative ) Acid aerobically from D _ Trehalose (positive) Fluorescent pigment ( negative ) Non fluorescent pigment (positive )

  25. P. Saccharophila Gelatin hydrolysis (P) Starch hydrolysis (P) Acid aerobically from D_ Trehalose (P ) Fluorescent pigment ( N ) Non fluorescent pigment ( N )

  26. P. Putida Gelatine hydrolysis ( negative ) Starch hydrolysis (negative ) Acid aerobically from D_ Trehalose (N ) Fluorescent pigment ( P ) Non fluorescent pigment ( P )

  27. The End

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