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Microscopes

Microscopes. Review. Magnification of Objectives . Field View/Field Diameter. Field of view is the area that you see when looking through the microscope Field Diameter is the measurement of the d istance from one end o f the field of view to t he other . Measuring Field Diameter.

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Microscopes

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  1. Microscopes Review

  2. Magnification of Objectives

  3. Field View/Field Diameter • Field of view is the area that you see when looking through the microscope • Field Diameter is the measurement of the distance from one end of the field of view to the other

  4. Measuring Field Diameter • You can measure the field diameter using the low power lens using a transparent ruler What you see here is measured in mm. To convert this to micrometers you must multiply by 1000. 1mm=1000 um

  5. Calculating Field Diameter at the High Powered Lens • Using the measured field diameter at the low powered lens we can calculate the field diameter at the high powered lens using the following formula. Field diameter (HP)= Field Diameter (LP) x Magnification (LP) Magnification (HP) As you increase the magnification the field diameter gets smaller

  6. Estimating the Size of Specimens • Using the field diameter you can estimate the size of specimens. By determining how many of the specimen would fit across the field diameter you can judge the size of the specimen. The creature on the right fits half way across a field diameter of 1000 um. This tells us the creature is 500 um.

  7. Magnifications of Drawings • If you draw your specimen it is important to label the magnification of the drawing This caterpillar is 20cm long in the picture but in reality it is only 5cm long. To find how much the picture magnifies the caterpillar You can use the following formula: Magnification= Size on drawing of drawing actual size 20cm/5cm= magnification of 4x

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