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Meristem Culture of Actinidia: Growth and Viability Study

This research presented at the International Student Symposium "Hortus Academicus" in Bucharest, Romania, investigates the meristem culture of Actinidia species. The study includes materials and methods, results, discussions, conclusions, and recommendations based on the remarkable growth and viability observed. References on kiwifruit micropropagation are also provided.

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Meristem Culture of Actinidia: Growth and Viability Study

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  1. Meristemculture of Actinidiasp. Authors names: Anca Barcu Coordinator Professor: Adrian Peticila International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

  2. Introduction • Actinia deliciosa (A) • Actinidachinensis (C) • Actinidiaarguta (D) International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

  3. Materials and Methods • Meristem culture • 52 meristems • 4 types of medium International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

  4. Results and discussions • -remarkable growth on medium with IBA, GA3 and ABA, 3 days dark > 7 days dark • -high viability on the same medium • -viable meristems after 5 days in sterilized water vs 7 days in sterilized water at 5C International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

  5. Conclusions and recommendations • -the best medium for growth is also the best medium for inoculation • -actinidiaargutagrows much faster than chinensis and deliciosa International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

  6. References • Micropropagation of kiwifruit using non-axenic shoot tips, PAUL L. MONETTE • Akbaş, Filiz & Işıkalan, Çiğdem & Namli, Süreyya & Başaran, Davut. (1560). Micropropagation of kiwifruit (Actinidiadeliciosa). INTERNATİONAL JOURNAL OF AGRİCULTURE & BİOLOGY. 8530. 9-3. • Micropropagation of Kiwifruit (Actinidiadeliciosa) FİLİZ ADIYAMAN AKBAŞ, ÇİĞDEM IŞIKALAN, SÜREYYA NAMLI1 AND DAVUT BAŞARAN • Rugini E., Gutiérrez-Pesce P. (2003) Micropropagation of Kiwifruit (Actinidia spp.). In: Jain S.M., Ishii K. (eds) Micropropagation of Woody Trees and Fruits. Forestry Sciences, vol 75. Springer, Dordrecht • Revilla M.A., Rey M.A., Gonzalez-Rio F., Gonzalez M.V., Diaz-Sala C., Rodriguez R. (1992) Micropropagation of Kiwi (Actinidia spp.). In: Bajaj Y.P.S. (eds) High-Tech and Micropropagation II. Biotechnology in Agriculture and Forestry, vol 18. Springer, Berlin, Heidelberg • Wessels E, Nel DD, Von Staden DFA (1984) In vitro propagation of Actinidiachinensis PI. Cultivar Hayward. Deciduous Fruit Grower 34: 453–457 • Tsai C-K (1988) Plant regeneration from leaf callus protoplasts of ActinidiachinensisPlanch, var. chinensis. Plant Sci 54: 231–250 International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

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