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Post Translational Modification

Post Translational Modification. Zachry Theis Clarity Argall. Overview. Post Translational Modification Glycosylation Tyrosine Sulfonation. Glycosylation. Description Purpose of Glycosylation Mechanism Types of Glycosylation. Description. Post Translational Modification

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Post Translational Modification

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  1. Post Translational Modification Zachry Theis Clarity Argall

  2. Overview • Post Translational Modification • Glycosylation • Tyrosine Sulfonation

  3. Glycosylation • Description • Purpose of Glycosylation • Mechanism • Types of Glycosylation

  4. Description • Post Translational Modification • Enzymatic Process • Carbohydrate Attachment • Found frequently in ER and golgi

  5. Structural Components Protein Folding Specific Recognition Endogeneous Recogoniton Exogeneous Recognition Purpose

  6. Mechanism • α linkage • β linkage

  7. N-Linked O-linked C-Linked Types of Glycosylation • Glycation

  8. N-Linked • Largely In Eukaryotes • Addition to Asparagine • Oligosaccharyltransferase • High Mannose Oligosaccharides • Complex Oligosaccharides

  9. O-Linked • After N-glycosylation • Golgi • Serine and Threonine • UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase

  10. C-Linked • Mannose to Tryptophan • Less common • Thrombospondins

  11. Glycation • Non Enzymatic • Endogeneous or Exogeneous • Impairs function • Alzheimer’s and Parkinson

  12. Tyrosine Sulfation

  13. Tyrosine Sulfation • Posttranslational Modification • Sulfate group added to Tyrosine residue of protein • Occurs in Golgi Apparatus Tyrosine

  14. Tyrosylprotein sulfotransferase Catalyst

  15. Mechanism

  16. 3'-Phosphoadenosine-5'-phosphosulfate

  17. Function • Strengthens Protein-Protein Interactions • Examples: • Serine Protease Inhibitors • Adhesion Molecules • Coagulation Factors

  18. References Alberts, Bruce. Molecular Biology of the Cell. New York: Garland Science, 2008. Print "Glycosylation." UniProt. UniProt, 20 July 2011. Web. 19 Nov. 2011. <http://www.uniprot.org/manual/carbohyd>. Varki, Ajit. Essentials of Glycobiology. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 2009. Print

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