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Protozoans. Protozoan Groups. Apicomplexans. Amebas. Ciliates. Flagellates. Newly Revised Protozoan Taxonomy. Clade Viridiplantae Phylum Chlorophyta algae with chlorophyll Phylum Euglenozoa euglenoidea kinetoplasta Clade Retortamonada no mitochondria Clade or Superphylum Alveolata
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Protozoan Groups Apicomplexans Amebas Ciliates Flagellates
Newly Revised Protozoan Taxonomy • Clade Viridiplantae • Phylum Chlorophyta • algae with chlorophyll • Phylum Euglenozoa • euglenoidea • kinetoplasta • Clade Retortamonada • no mitochondria • Clade or Superphylum Alveolata • Phylum Apicomplexa • Phylum Ciliophora • Phylum Dinoflagellata • Amebas
Basis of Taxonomy Morphological traits Flagella Cilia Pseudopodia Shells (tests) Reproductive modes Sexual Asexual Spore formation Distinct reproductive cell forms Cyst formation Characteristic organelles Apical complex Kinetoplast mitochondria plastids Trophism Heterotrophic Autotrophic Molecular DNA sequences gene sets
Symbiotic Relationships • Host • Symbiont • Relationship • Parasitism • Commensalism • Mutualism
Flagella & Cilia • Axoneme • Kinetosome
Amoeba • Amoeboid movement • Pseudopodial extension • Phagocytosis • Engulfment of large particles
Contractile Vacuoles • Osmotic regulation • Remove excess metabolic H2O, and HCO3 • Remove H2O that enters by osmosis
Sexual Reproduction • Conjugation • Meiotic nuclear division • Transfer of portion of genome between partners • Sporogomy • Formation of sporozoites • Formation of gametocytes from sporozoites • Fusion of gametocytes to form new generation of sporozoite • Gametocytes and sporozoites are present in separate hosts
Euglenozoa • A phytoflagellate • Autotrophic • Opportunistic heterotroph
Free Living Flagellates Pandorina colony
Colonial Flagellates Volvox colony
Flagellates • Parasitic examples • Cyst formers
Parasitic Kinetoplastids • Phylum Euglenozoa • Subphylum Kinetoplasta • Class Trypanosomatidea • Trypanosoma spp • Leishmania spp
Trypanosomiasis • African sleeping sickness – Eastern hemisphere • Trypanosoma brucei • Vector – Tsetse Fly - Glossina spp Immages from links at http://www.biosci.ohio-state.edu/~parasite
Trypanosomiasis • Chaga’s disease – Western hemisphere • Trypanosoma cruzi • Vector – assassin bug – Rhodnius spp, Panstrongylus spp and Triatoma spp
Life Cycle of Trypanosoma • http://www.genomics.liv.ac.uk/tryps/trypsmovie.html
Leishmaniasis • Leishmania spp • Vector – sandfly – OW Phlebotomus spp, NW Lutzomyia, Brumptomyia • Similar protozoan to Trypanosoma sp • Cutaneous and visceral forms of disease
Taxonomy of Apicomplexans • Phylum Apicomplexa • Class Coccidiea • Plasmodium spp , Toxoplasma spp • Life cycle with multiple hosts • Class Gregarinea • Gregarines – invertebrate parasites • Single host life cycles
Life Cycle of Plasmodium vivax Anopheles spp
Phylum Ciliophora Tetrahymena Vorticella Stentor
Reproduction in Ciliates Conjugation Fission
Conjugation derived from the micronucleus.
Reproduction in Ciliates – Sexual and Asexual Fission = asexual
http://youtu.be/rqD3m9hHhlo http://youtu.be/pds8w7C9FEw