1 / 12

Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection

Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection. By Yujun Song, Konggang Qu, Chao Zhao, Jinsong Ren, and Xiaogang Qu*. Introduction. 1.GN 的研究现状使其在医药诊断和生物技术方面的应用成为可能; 2. 本文提出 GO-COOH 有类似过氧化物酶的作用,并简介研究成果。. 1. 提出过氧化物酶作为 H2O2 和葡萄糖诊断试剂的应用还有广阔的发展空间;

rigg
Download Presentation

Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection By Yujun Song, Konggang Qu, Chao Zhao, Jinsong Ren, and Xiaogang Qu*

  2. Introduction 1.GN的研究现状使其在医药诊断和生物技术方面的应用成为可能; 2.本文提出GO-COOH有类似过氧化物酶的作用,并简介研究成果。 1.提出过氧化物酶作为H2O2和葡萄糖诊断试剂的应用还有广阔的发展空间; 2.简介葡萄糖传感器的研究现状(特别强调了下HRP),并将本文中的GO-COOH和HRP进行了简单的对比。 Simple Cheap highly sensitive highly selective

  3. Catalytic mechanism

  4. Experimental Expandable graphite flake + NaCl ground 10min H2O2+GO+TMB H2O UV-vis filtration Glucose+GOx+GO+TMB Hummer’s method AFM FTIR EDX GO-COOH [1] [1] X. M. Sun, Z. Liu, K. Welsher, J. T. Robinson, A. Goodwin, S. Zaric, H. Dai, Nano Res. 2008, 1, 203.

  5. Results

  6. Results

  7. Results Ping-pong mechanism

  8. Results [LUMO] Electron transfer mechanism

  9. Results

  10. Results

  11. Conclusion • 1 GO-COOH possesses intrinsic peroxidase-like activity and its catalysis is strongly dependent on pH, temperature, and H2O2 concentration, similar to HRP. • 2 In the presence of H2O2 and peroxidase substrate TMB, GO-COOH can produce a blue color reaction. Kinetic analysis indicates that the catalysis follows a ping-pong mechanism, and GO-COOH has even higher catalytic activity to TMB than HRP. • 3 GO-COOH, as a mimic peroxidase, shows several advantages over natural enzymes, such as ease of preparation, low-cost, and stability. • 4 Compared to HRP and Fe3O4 nanoparticles, GO-COOH possesses high surface-to-volume ratios as well as high affinity to organic substrates through π-π and hydrophobic interactions. • 5 We design and develop a simple, cheap, and highly selective and sensitive colorimetric assay to detect glucose in buffer solution or diluted blood and fruit juice samples. Our work will facilitate the utilization of GO-COOH intrinsic peroxidase activity in medical diagnostics and biotechnology.

  12. Thank You !

More Related