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Forensic Biology by Richard Li

Chapter 12: DNA Extraction. Forensic Biology by Richard Li. Basic Principles. Vary according to type of biological evidence Method of choice= yields sufficient quantity, good quality, and high purity of DNA Insufficient quantity Poor quality Low purity

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Forensic Biology by Richard Li

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  1. Chapter 12: DNA Extraction Forensic Biologyby Richard Li

  2. Basic Principles • Vary according to type of biological evidence • Method of choice= yields sufficient quantity, good quality, and high purity of DNA • Insufficient quantity • Poor quality • Low purity • Most common methods involve isolating total cellular DNA Failure to obtain profile or partial profile Forensic Biology by Richard Li

  3. Basic Principles • Components: • Cell & Tissue Disruption • Lysis of Membranes & Organelles • Removal of Proteins & Cytoplasmic Contaminants • Contamination • Storage Forensic Biology by Richard Li

  4. Basic Principles • Cell & Tissue Disruption • Proteinase K • Boiling • Alkali treatment Forensic Biology by Richard Li

  5. Basic Principles • Lysis of Membranes & Organelles • Detergents • Buffer • High Salt concentrations • Reducing agents • Chelating agents Forensic Biology by Richard Li

  6. Basic Principles • Contamination • Introduction of exogenous DNA • Person and sample • Sample to sample • Samples and amplified DNA • Material from other organisms • Reagent blanks • Process separately • Use approved procedures Forensic Biology by Richard Li

  7. Methods for Extraction • Extraction with Phenol-Chloroform • Organic extraction • Extraction by Boiling Lysis & Chelating Agent • Chelex extraction method • Silica-based DNA Extraction • Chaotropic salts • Differential Extraction • Sexual assault cases Forensic Biology by Richard Li

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