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Evolution at the Nucleotide Level

Evolution at the Nucleotide Level. Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans. Matthew D. Pagel NSCORT/Exobiology Fellow Christopher Wills Lab Department of Biology, University of California - San Diego. HIV/SIV Phylogenetic Trees. A Model for Rapid Evolution.

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Evolution at the Nucleotide Level

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  1. Evolution at the Nucleotide Level Rapid Evolution of HIV/SIV and Microsatellites in Candida albicans Matthew D. Pagel NSCORT/Exobiology Fellow Christopher Wills Lab Department of Biology, University of California - San Diego

  2. HIV/SIV Phylogenetic Trees A Model for Rapid Evolution

  3. Sample Sequence Alignment

  4. HIV/SIV Env gene Neighbor-Joining Tree(from Yellow Fever, Black Goddessby C. Wills)

  5. Neighbor-Joining Tree of Env gene of HIV/SIV - NCBI/Los Alamos Complete Sequences

  6. Pol Neighbor-Joining Tree HIV1 HIV2

  7. Neighbor-Joining Tree of Gag Gene of HIV/SIV

  8. NJ Tree of Gag after Unverified Sequences Removed

  9. Cloning of Candida albicans genes involved in pathogenicity Plasmid Construction of Erk1

  10. Microsatellite Slippage Mutations (Glutamine)

  11. The Genetic Code--The Translation of mRNA to Amino Acids--

  12. Recombination-Mediated Plasmid Construction

  13. Action of Restriction Enzymes(Eco RI example clipped from Molecular Cell Biology CD-Rom)

  14. Plasmid Constructionvia Restriction Digest

  15. Blue/White Screen

  16. Future Steps • Site-directed mutagenesis producing variant microsatellite length • Re-insertion of plasmid into knockout strains • Analysis of morphological form (hyphal)

  17. Acknowledgements Chris Wills Nikki Freed Dave Metzgar Sol Guiterrez Hattie Oswald Lois Lane Bill Baity Carlos Flores, Vincent Starai, Carlos Peralta, & William Engels The NSCORT/Exobiology Program

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