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Supplemental Figure 1. F(ab’)2 fragments from patient-derived ANCA IgG do not induce neutrophil respiratory burst and degranulation. A. B. C. D. 24 hours. 1 2 3 4 5 6. 1 2 3 4 5 6. kDa. Stain. LCA. GST. EndoS. 7 days. 7 days.
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Supplemental Figure 1. F(ab’)2 fragments from patient-derived ANCA IgG do not induce neutrophil respiratory burst and degranulation A B C D
24 hours 1 2 3 4 5 6 1 2 3 4 5 6 kDa Stain LCA GST EndoS 7 days 7 days 1 2 3 4 5 6 1 2 3 4 5 6 kDa 1 2 3 4 5 6 1 2 3 4 5 6 kDa Stain Stain LCA LCA GST EndoS GST EndoS 55 55 55 70 70 70 70 55 55 55 70 70 Supplemental Figure 2. Analysis of serum IgG deglycosylation after early (3h) and late (24h) EndoS treatment A B C
