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Aerobic Gram Negative Cocci

Aerobic Gram Negative Cocci. Student Lab Division of Medical Technology Jeanne Filbey, MT(ASCP). Aerobic GNDC. General Characteristics. Normal flora of upper respiratory, alimentary and genitourinary tracts Gram stain: GNDC Oxidase positive Catalase positive Non-motile. Fastidious

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Aerobic Gram Negative Cocci

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  1. Aerobic Gram Negative Cocci Student Lab Division of Medical Technology Jeanne Filbey, MT(ASCP)

  2. Aerobic GNDC General Characteristics • Normal flora of upper respiratory, alimentary and genitourinary tracts • Gram stain: GNDC • Oxidase positive • Catalase positive • Non-motile

  3. Fastidious Temperature sensitive Culture immediately Transport media if delay Aerobic GNDC Neisseria gonorrhoeae

  4. Neisseria gonorrhoeae Specimen Gram Stain • Intracellular GNDC

  5. Neisseria gonorrhoeae Colony Morphology • Chocolate agar • Thayer-Martinagar • Growth • Contains antibiotics

  6. Neisseria gonorrhoeae Identification • Carbohydrates • Glucose pos • Maltose neg • Lactose neg • Sucrose neg • DNase neg • Nitrate neg • Butyrate esterase neg

  7. Neisseria gonorrhoeae Pathogenesis • Port of entry • Progression of infection • Human to human transfer • Females asymptomatic

  8. Neisseria gonorrhoeae Gonorrhea • Male • Acute urethritis, prostatitis and epididymitis if untreated • Female • May be asymptomatic • Cervicitis --> pelvic inflammatory disease --> scarring of fallopian tubes --> sterility, ectopic pregnancies

  9. Neisseria gonorrhoeae Other Infections • Disseminated gonococcal infection • Pharyngitis • Gonococcal ophthalmia neonatorum • Vulvovaginitis

  10. Neisseria gonorrhoeae Treatment • Antimicrobial testing

  11. Fastidious Temperature sensitive Culture immediately Occasionally grows on SBA Aerobic GNDC Neisseria meningitidis

  12. Neisseria meningitidis Specimen Gram Stain • GNDC

  13. Neisseria meningitidis Colony Morphology • Chocolate agar • Thayer-Martinagar • Growth

  14. Neisseria meningitidis Identification • Carbohydrates • Glucose pos • Maltose pos • Lactose neg • Sucrose neg • DNase neg • Nitrate neg • Butyrate esterase neg

  15. Neisseria meningitidis Pathogenesis • Meningitis • Septicemia • Septic arthritis • Endocarditis • Pneumonia • Urethritis • Asymptomatic nasopharyngeal carriers

  16. Normal flora Aerobic GNDC Neisseria lactamica

  17. Neisseria lactamica Colony Morphology • Chocolate agar • Thayer-Martinagar • Growth

  18. Neisseria lactamica Identification • Carbohydrates • Glucose pos • Maltose pos • Lactose pos • Sucrose neg • DNase neg • Nitrate neg • Butyrate esterase neg

  19. Can be normal respiratory flora Aerobic GNDC Moraxella (Branhamella) catarrhalis

  20. Moraxella (Branhamella) catarrhalis Colony Morphology • Sheep blood agar • Chocolate agar • Thayer-Martinagar • Growth

  21. Moraxella (Branhamella) catarrhalis Identification • Carbohydrates • Glucose neg • Maltose neg • Lactose neg • Sucrose neg • DNase pos • Nitrate pos • Butyrate esterase pos

  22. Moraxella (Branhamella) catarrhalis Pathogenesis • Acute bronchitis • Pneumonia • Meningitis • Seen in immunocompromised patients (elderly) • Otitis media & maxillary sinusitis in kids • Most produce beta lactamase

  23. Aerobic GNDC Summary • Specimen handling and processing • Growth characteristics • Identification • Clinical significance • Antimicrobial testing

  24. Who am I? Urethral gram stain Thayer Martin agar Neisseria gonorrhoeae

  25. Who am I? CSF gram stain Chocolate agar Neisseria meningitidis

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