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Structural Variation in the Human Genome Michael Snyder March 2, 2010

Structural Variation in the Human Genome Michael Snyder March 2, 2010

Structural Variation in the Human Genome Michael Snyder March 2, 2010. Single nucleotide polymorphisms (SNPs). GATTTAGATC G CGATAGAG GATTTAGATC T CGATAGAG. Genetic Variation Among People. 0.1% difference among people. Mapping Structural Variation in Humans. >1 kb segments.

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1.06k views • 80 slides
southgreen.cirad.fr/

southgreen.cirad.fr/

Introduction, presentation of the Southgreen platform. http://southgreen.cirad.fr/. Manuel Ruiz, Bioinformatics School, Campinas, Sao Paulo, Brazil, 21-26 november 2011. Montpellier. The impact of NGS. Today within labs, bioinformaticians can perform a comprehensive analysis of

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676 views • 40 slides
Yaniv Erlich Hannon Lab

Yaniv Erlich Hannon Lab

shRNA libraries sequencing using DNA Sudoku. Yaniv Erlich Hannon Lab. Preparing DNA libraries. Programmable microarray. Cloning into plasmids. Transformation. Array single colonies. The problem. Input : 40,000 bacterial colonies Output: The sequence of the shRNA inserts. Insert type.

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410 views • 25 slides
Sequencing technologies and Velvet a ssembly

Sequencing technologies and Velvet a ssembly

Sequencing technologies and Velvet a ssembly. Lecturer ? Du Shengyang September 29 ? 2012. The Advances of DNA Sequencing Technology. ?????. Sanger ?. ????????. 454. The second generation of sequencing technologies. Solexa. SOLiD.

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426 views • 23 slides
Solexa & Lab IT Infrastructure January 8, 2008: Nelson Lab Meeting Jordan Mendler

Solexa & Lab IT Infrastructure January 8, 2008: Nelson Lab Meeting Jordan Mendler

Solexa & Lab IT Infrastructure January 8, 2008: Nelson Lab Meeting Jordan Mendler Nelson Lab, UCLA Human Genetics Department jmendler@ucla.edu. Cluster Configuration: Queues. all.q : The main production queue.

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356 views • 19 slides
RNA-Seq

RNA-Seq

RNA-Seq. An alternative to microarray. Grow cells or isolate tissue (brain, liver, muscle) Isolate total RNA Isolate mRNA from total RNA (poly A+ select) Fragment RNA Make and amplify cDNA Sequence the ends of the cDNA Map the sequences to the human genome

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398 views • 15 slides

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