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AO-Biotics® Egg Equality Enhancer (EQE) BioZyme, Incorporated
Executive Summary ▪ AO-Biotics® EQE is the first-and-only AO postbiotic developed specifically for laying hens. ▪ Key Benefits 1. More sellable eggs 2. Improved egg mass 3. Increased productive life span
Postbiotic Definition https://isappscience.org/behind-the-publication-understanding-isapps-new-scientific-consensus-definition-of- postbiotics/
Expectations with AO-Biotics EQE • 3% more egg mass • (summary from 5 validation trials, 330 thousand hens) • 2% improvement in egg production • (summary from 6 validation trials, 390 thousand hens) • 3% more sellable egg per hen • (summary from 5 validation trials, 330 thousand hens) • 27% less mortality • (summary from 6 validation trials, 314 thousand hens) TIER 1
Expectations with AO-Biotics EQE MORE Egg Mass BETTER Egg Production LESS Mortality Control MORE Eggs × Hen MORE Eggs Sold AO-Biotics EQE® Validation 1 = Italy validation 24-41 weeks of age (40,000 hens) / Validation 2 = Italy validation 42-60 weeks of age (40,000 hens) / Validation 3 = Mexico 14-45 weeks of age (80,000 hens) / Validation 4 = Mexico 15-51 weeks of age (70,000 hens) / Validation 5 = France 22-45 weeks of age (control means average from Off periods 22-30 and 40-45 weeks of age, and AO.biotics EQE® average is 31- 39 weeks of age; 100,000 hens) / Validation 6 = Germany 26-45 weeks of age (24,000 hens) / Validation 7 = Germany 26-88 weeks of age (control is the average of the standard curve of the bred used; 60,000 hens) • For the number of sellable eggs, results obtained during the validation periods were used, standardizing the number of animals in each of the houses used and the weeks of production to 20 thousand animals per house and 18 weeks of production, respectively. This makes it possible to better evaluate the impact of the postbiotic AO on egg production in laying hens. TIER 2