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Develop a Bandwidth Detection System to Monitor Metabolic Activity of Single Cells

Develop a Bandwidth Detection System to Monitor Metabolic Activity of Single Cells. Jerry Wilmink and Jonathon Wells Advisor: Franz Baudenbacher BME 273, Group 9. Project Inception?. BioMEMS Device Funded by DARPA Cell based sensing technology Monitor Cell Metabolism

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Develop a Bandwidth Detection System to Monitor Metabolic Activity of Single Cells

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  1. Develop a Bandwidth Detection System to Monitor Metabolic Activity of Single Cells • Jerry Wilmink and Jonathon Wells • Advisor: Franz Baudenbacher • BME 273, Group 9

  2. Project Inception? • BioMEMS Device • Funded by DARPA • Cell based sensing technology • Monitor Cell Metabolism • Subnanoliter volumes

  3. BioMEMS • Fabricated on Silicon Chip • Peristaltic Pumps • Fluidics Layer • Interdigitated Array electrodes

  4. Expected Acidification Rate of a Single Cell in a 0.1 nL Volume BioMEMS Combine electrodes with flourescence probes Quantify and Classify the impact of various toxins on cell physiology CBW Agents to develop signatures

  5. Project Overview Step 1: Design the hardware for a 256 channel data acquisition system to read out the Hamamatsu photodiode array

  6. Project Overview Step 2: Design Labview program to control the data acquisition system and provide control signals for the valves driving the bioMEMS device

  7. Project Overview Step 3: Design Hardware to interface the photodiode array with an inverted microscope and the bioMEMS devices

  8. Project Overview Step 4: Devise a calibration protocol for the photodiode array bandwidth of dye

  9. Project Overview Step 5: Record transmembrane potential of single cardiac myocytes with flourescent probes and record from electrochemical sensors

  10. Progress Research Darpa Proposal Journals, Internet etc Began Step 1: Install Labview Install NI data acquisition board Soder individual channel to corresponding photo diode Apply current to bioMEM to make sure it worked

  11. Future Plans Begin Step 2: Design Labview program That enables specific channel identification That is read in from specific photodiodes Work with potentiostat readout and manipulate and quantify results

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