1 / 8

DNA Identification

Ch 9-1 & 9-2. DNA Identification. The repeating sequences in noncoding DNA vary between individuals and thus can be used to identify an individual. Steps in DNA Identification. Copying DNA

sills
Download Presentation

DNA Identification

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Ch 9-1 & 9-2 DNA Identification • The repeating sequences in noncoding DNA vary between individuals and thus can be used to identify an individual.

  2. Steps in DNA Identification • Copying DNA • To identify a DNA sample, scientists isolate the DNA and copy it using thePolymerase Chain Reaction (PCR) • Millions of copies are made in just a few hours

  3. Polymerase Chain Reaction

  4. Steps in DNA Identification • Cutting DNA • The DNA is then cut into fragments using restriction enzymes • Restriction enzymes recognize and cut specific nucleotide sequences called a restriction site.

  5. Restriction Enzymes Cut DNA

  6. Steps in DNA Identification • Sorting DNA by Size • The fragments are separated by size using gel electrophoresis • A positive electrode is attached to one side of the gel (a thin slab of hard gelatin) • A negative electrode is attached to the other side • An electric current is sent through the gel and the DNA (which is slightly negative) moves towards the positive side

  7. Steps in DNA Identification • Sorting DNA by Size • There are pores in the gel for the DNA to move through • Large pieces of DNA stop sooner (closer to the negative end) • Small pieces of DNA move farther (closer to the positive end) • The resulting pattern of bands is called a DNA fingerprint • a Restriction map shows the lengths of the bands produced in the gel

  8. Gel Electrophoresis

More Related