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An Examination of New Turfgrass Insecticides and Their Effects on Nontarget Arthropods in Turf

Dan Digman Research Technician The Ohio State University Department of Entomology Columbus, OH digman.6@osu.edu. An Examination of New Turfgrass Insecticides and Their Effects on Nontarget Arthropods in Turf. Evaluate Impact of New Insecticides on Nontarget Arthropods in Turfgrass:.

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An Examination of New Turfgrass Insecticides and Their Effects on Nontarget Arthropods in Turf

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  1. Dan Digman Research Technician The Ohio State University Department of Entomology Columbus, OH digman.6@osu.edu An Examination of New Turfgrass Insecticides and Their Effects on Nontarget Arthropods in Turf

  2. Evaluate Impact of New Insecticides on Nontarget Arthropods in Turfgrass: • Halofenozide (=MACH2TM) • Imidacloprid (=MeritTM) • Deltamethrin (= DeltaGardTM) • Indoxacarb (=StewardTM) • Thiamethoxam (= MeridianTM)

  3. Objectives • Design and conduct a nontarget study to evaluate effects of halofenozide, imidacloprid, deltamethrin, indoxacarb and thiamethoxam on common arthropods in turf • Compare data obtained from previous nontarget studies using insecticides currently on the market • Determine the preliminary biodiversity in turfgrass

  4. Rate Insecticide lbAI / acre LD50* Class Product Imidacloprid 0.3 450 Neonicotinoid Merit 75WP Halofenozide 2.0 2850 IGR MACH2 22.3EC Thiamethoxam 0.2 1563 Neonicotinoid Meridian 75WDG Deltamethrin 0.05 135 Pyrethroid DeltaGard 5SC Indoxacarb 0.13 >5000 Oxadiazone Steward Treatments Used in Study * Rat oral LD50 for technical, all formulations were >5000

  5. Application Method • Five replications of six treatments with 0.61 meters between plots • Plots (3.05 m2) arranged in an RCB (Random Complete Block) Design • A Tee-Jet triple-boom CO2 spray tank with 8004 nozzles was callibrated to deliver 2 gal/1,000 ft2 at 20 psi • Experimental area established at The OSU-OTF Turfgrass Research Facility in Columbus, Ohio

  6. Treatment and Collection Dates • Samples include a single pre-treatment core taken per plot on 15 July, 2002 • Plots were treated on 22 July, 2002 • After treatment date, two turf samples were collected per plot at 1, 2, 3, 4, 6, 11, 25 and 27 weeks after treatment (WAT)

  7. Turf Core Extractions • Samples were inverted (turf-side down) into Berlese funnels for arthropod extraction • Then capped and stored in 80% ethanol until analysis

  8. Statistical Analysis • Data were analysed using MINITAB (Minitab, Inc., State College, PA) • ANOVA multi-comparisons were calculated and with a Tukey mean separation using error rate of 5% (when valid).

  9. Evaluation of 2002 Data • There was an average of 27,318 arthropods per m2 in the pretreatment samples. • Of those, 80% were Acari and 14% were Collembola. • Preliminary analysis indicated insufficient data to consider Aranae, Hemiptera, Coleoptera, Hymenoptera, Diptera, Thysanoptera and Lepidoptera as valid indicator groups. • Analysis focused on Acari and Collembola

  10. 2002 Mite Data

  11. 2002 Collembola Data

  12. 2002 Diplura Data

  13. Back to the Drawing Board... • Establish two additional studies mirroring the 2002 experiment with four replications and six treatments each • Take data at 1, 2, 3, 4 and 6 WAT to observe correlations or differences

  14. 2005 Data: Total Acari

  15. Acari: Break-Down

  16. Preliminary Data: Collembola

  17. Collembola: Break-Down

  18. Collembola: Continued...

  19. Preliminary Data: Diplura

  20. Diplura: Break-Down

  21. Control Plot Total ArthropodComparison 2002 vs. 2005

  22. Preliminary Conclusions • Data support no significant effects on families of Acari within first four weeks following treatments... • Data for Collembola show significant effects for Sminthuridae by all treatments at 1 WAT. • Data suggests that Diplura are not significantly affected by any treatments. • Unknown carrying capacity may be linked to seasonal organic matter content in turfgrass.

  23. Biodiversity Data • Peck et al. 2001 Biodiversity Data • Digman et al. 2002 Biodiversity Data

  24. Discovery is everything... Thank you...

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