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Supplementary Fig. 1a. atpD

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Supplementary Fig. 1a. atpD

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  1. Supplementary Fig. 1. Comparison of atpD (a), recA (b) and glnII (c) gene phylogenies, showing the relationships among the chickpea rhizobia in the present study and recognized species of the genus Mesorhizobium. Trees were constructed by the neighbour-joining method with a Kimura 2-parameter model distance matrix. Bootstrap values (%) were based on 1,000 replications and were shown at each node. Bars, expected number of changes per site. Other ten sequences of atpD, recA or glnII gene respectively identical with that of CCBAU 83963 presented in the relevant phylogenic tree were also obtained and deposited under the collection and accession numbers (atpD, recA and glnII) of CCBAU 83980 (HQ316727, HQ316785, HQ316742), CCBAU 83978 (HQ316725, HQ316783, HQ31674), CCBAU 83953 (HQ316723, HQ316781, HQ31673), CCBAU 83948 (HQ316722, HQ316780, HQ31673), CCBAU 83943 (HQ316721, HQ316779, HQ316736), CCBAU 83935 (HQ316719, HQ316777, HQ316734), CCBAU 83923 (HQ316718, HQ316776, HQ316733), CCBAU 83919 (HQ316717, HQ316775, HQ316732), CCBAU 83915 (HQ316716, HQ316772, HQ316731), CCBAU 83994 (HQ316728, HQ316786, HQ316743). Supplementary Fig. 1a. atpD

  2. Supplementary Fig. 1b. recA

  3. CCBAU 831015 (HQ316729) 100 CCBAU 83979 (HQ316741) CCBAU 83908 (HQ316730) 98 CCBAU 83939 (HQ316735) 61 CCBAU 83963 (HQ316739) 55 0.01 M.temperatum SDW 018T (DQ345073) 93 M. mediterraneum USDA 3392T (AF169578) 96 M. robiniae CCNWYC 115T (JN202309 ) 65 M.gobiense LMG23949T(EF549451) M. tarimense CCBAU 83306T(EF549452) 98 M.tianshanense USDA 3592T(AF169579) M.metallidurans LMG24485T(HM047123) M.caraganae CCBAU 11299T(EU249384) M. amorphae ACCC 19665T (EU518372) 98 100 M. septentrionale SDW014T (EU249387) M.ciceri USDA 3383T (AF169580) 65 M.australicum LMG 24608T (JN202307 ) M.opportunistum 24607T (HM047122) M. loti LMG 6125T (AF169581) 97 100 M. plurifarium ICMP 13640T (AY494794) M. camelthorni CCNWXJ40-4T (JN202308 ) M. huakuii USDA 4779T (AF169588) 92 M.shangrilense LMG24762T(EU672486) M. chacoense ICMP 14587T(AY494791) 64 M.albiziae CCBAU 61158T(DQ311091) R.leguminosarum USDA 2370T(EU155089) 100 R.fabae CCBAU 33202T(EF579935) Supplementary Fig. 1c. glnII

  4. CCBAU 83923 (HQ316760) 95 CCBAU 83948 (HQ316764) CCBAU 83919 (HQ316759) CCBAU 83935 (HQ316761) CCBAU 83979 (HQ316769) 85 CCBAU 83978 (HQ316768) CCBAU 83980 (HQ316770) CCBAU 83953 (HQ316766) CCBAU 83951 (HQ316765) 66 CCBAU 83963 (HQ316767) 0.2 CCBAU 83943 (HQ316763) CCBAU 831015 (HQ316757) Supplementary Fig. 2. Phylogenies of symbiotic gene nifH showing the relationships of the bacteria isolated from nodules of chickpea in Xinjiang. Rhizobium hainanense CCBAU 57015T was used as outgroup. The tree was constructed using neighbor-joining method. Scale bars indicate 0.2 substitutions of nucleotide. Bootstrap values greater than 50% were indicated at node. 95 CCBAU 83908 (HQ316758) 51 CCBAU 83939 (HQ316762) CCBAU 83994 (HQ316771) M. ciceriUSDA 3378T(EU267715) 87 M. mediterraneumUSDA 3392T(EU267716) M. chacoense LMG 19008T (DQ450927) M. plurifarium LMG 11892T (EU267717) M. septentrionale SDW 014T (EU130411) M. caraganae CCBAU 11299T (EU130422) M. shangrilense CCBAU 65327T (EU877532) M. albiziae CCBAU 61158T (DQ311093) M. amorphae ACCC 19665T (EU267714) M. tianshanense CCBAU 3306T (EU267718) // M. temperatum SDW018T (EU130410) M. loti NZP 2213T (DQ450929) R. hainanenseCCBAU 57015T (AY934876)

  5. Supplementary Fig. 3. Dendrogram based on BOX-fingerprints showing the diverse populations of the 42 isolates representing different IGS-RFLP types and sampling sites. Dice coefficient and the unweighted pair grouping with mathematic average (UPGMA) method were used for cluster analysis.

  6. Hae III Hha I Msp I 100 60 40 80 Type 1 (M. loti NZP 2213T ) Type 2 (M. cicer USDA 3378T) Type 3 (M. caraganae CCBAU 11299T) (CCBAU 831015 CCBAU 83915 CCBAU 83919 CCBAU 83923 CCBAU 83935 CCBAU 83943 Type 4 CCBAU 83953 CCBAU 83963 CCBAU 83948 CCBAU 83978 CCBAU 83994 CCBAU 83980 ) Type 5 (CCBAU 83939) Type 6 (M. temperatum SDW 018T) Type 7 (M. mediteraneum USDA 3392T ) Type 8 (CCBAU 83908) Type 9 (M. gobiense CCBAU 83330T) Type 10 (M. tarimense CCBAU 83306T ) Type 11 (M. amorphae ACCC 19665T ) Type 12 (M. huakuii CCBAU 2609T ) Type 13 (M. septentrionale SDW 014T ) Type 14 (M. tianshanese CCBAU 3306T) Type 15 (M. albiziae CCBAU 61158T) Supplementary Fig. 4. Dendrogram of IGS-RFLP showing the grouping results of chickpea rhizobia and their relationships with the reference strains.

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