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X-ray microanalysis of heavy metals in lung

X-ray microanalysis of heavy metals in lung. Research training report 2005. Han Li. Introduction. Department of Medical Cell Biology, BMC Supervisor: Prof. Godfried M. Roomans Project: * literature study on the X-ray microanalysis of metals in lung

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X-ray microanalysis of heavy metals in lung

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  1. X-ray microanalysis of heavy metals in lung Research training report 2005 Han Li

  2. Introduction • Department of Medical Cell Biology, BMC • Supervisor: Prof. Godfried M. Roomans • Project: * literature study on the X-ray microanalysis of metals in lung * analyze lung specimen from a dental technician • Technique: Energy dispersive X-ray (EDX) microanalysis • Instrument: Transmition Electron Microscope (TEM)

  3. Background (Ⅰ) • Heavy metal toxicity (acute & chronic) • Respirotary system is most sensitive to heavy metal influence

  4. Background (Ⅱ) • Occupational exposure & Therapy introduced Occupational exposure Sn, As, Cu, Al, … - miners Ti - painters Fe - welders … Therapy introduced Ti - implants Ga - NMR contrasting media W & Sb - drug HPA-23 …

  5. Background (Ⅲ) • Early detection • EDX microanalysis is becoming the most favored method non-destructive, fast, capable of multi-elemental detection at trace quantities

  6. Materials and Methods • The lung specimen from: an old local dental technician suffering from dyspnea prepared by: Department of Pathology, Linköping University Hospital procedure: fixed in glutaraldehyde  embedded in epoxy plastic  sectioned • Electron microscopy Hitachi H-7110 TEM: accelerating voltage: 75 kV, 5000 x • EDX microanalysis Hitachi H-7110 TEM: STEM mode, accelarating voltage: 100 kV Oxford energy-dispersive Si(Li) X-ray detector: analysis time: 70 s • Data processing Link ISIS software: spectra collection (50)  automatic identification (indexing) element screen

  7. Results (Ⅰ) • Electron microscopy The morphology was not fully retained Nucleus is clear () Particles are clearly deposited () 2 µm

  8. Results (Ⅱ) • EDX microanalysis

  9. Conclusion • Heavy metals are detected in the lung specimen • The appearance of gold, zinc, and silver may be due to occupational exposure • Co-existence pattern can be found ( not highly evident)

  10. Acknowledgements • Professor Roomans • Leif Ljung • Department of Medical Cell Biology

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