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DNA Extraction

DNA Extraction. Common molecular biology lab procedure Why extract DNA ? To study DNA structure and function Paternity testing/Genetic testing Forensics. Key Players. Cells – DNA stored in nucleus Salt (NaCl) DNA has a negative charge Na + neutralizes DNA’s charge. Key Players.

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DNA Extraction

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  1. DNA Extraction • Common molecular biology lab procedure • Why extract DNA ? • To study DNA structure and function • Paternity testing/Genetic testing • Forensics

  2. Key Players • Cells – DNA stored in nucleus • Salt (NaCl) • DNA has a negative charge • Na+ neutralizes DNA’s charge

  3. Key Players • Detergent • dissolves lipids of membrane

  4. Remove Proteins • Enzyme added • Protease –breaks down the proteins • “cuts” histones from DNA http://learn.genetics.utah.edu/units/activities/extraction/enzyme

  5. Precipitate DNA • Ethanol added • DNA is insoluble in ethanol (clumps together) DNA ethanol cells/soap/salt http://learn.genetics.utah.edu/units/activities/extraction

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