1 / 5

Lab Exercise 14

taya
Download Presentation

Lab Exercise 14

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

    1. Lab Exercise 14 Gram Staining

    2. Background Gram Staining developed in the late 1800’s Gram Staining is a Differential staining technique that distinguishes between 2 broad groups of bacteria based on differences in the cell wall.

    4. Staining Procedure Place a slide with a bacterial smear on a staining rack. STAIN the slide with crystal violet for 1-2 min. Pour off the stain. Flood slide with Gram's iodine for 1-2 min. Pour off the iodine. Decolourize by washing the slide briefly with acetone (2-3 seconds). Wash slide thoroughly with water to remove the acetone - do not delay with this step. Flood slide with safranin counterstain for 2 min. Wash with water. **Gram Stain Animation

More Related