1 / 4

Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and Karyotyping of parental, vector-infected and TG2-transfected LNCaP cells (B). B. A. Relative cell growth over 3 days. LNCaP LNCaP -EV LNCaP -TG2.

tracey
Download Presentation

Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and Karyotyping of parental, vector-infected and TG2-transfected LNCaP cells (B) B A Relative cell growth over 3 days LNCaPLNCaP-EV LNCaP-TG2

  2. Fig. S2. Co-localization of TG2 and NF-κB in nuclear fraction of prostate cancer cells (A). Transient down regulation of NF-kB partially restored AR expression in PC3 cells (B). Putative NF-κB binding sites in the promoter of AR gene (C and D). p65 siR A B ContsiR p65 AR Actin LNCaP TG2 LNCaP Cont. LNCaP EV PC3 C Exon 1 -38 bp GACCCGACTCGCAAACTGTTGCATTTGCTCTCCACCTCCCAGCGCCCCCTCCGAGATCCCGGGGAGCCAGCTTG CTGGGAGAGCGGGACGGTCCGGAGCAAGCCCAGAGGCAGAGGAGGCGACAGAGGGAAAAAGGGCCGAGCTAG CCGCTCCAGTGCTGTACAGGAGCCGAAGGGACGCACCACGCCAGCCCCAGCCCGGCTCCAGCGACAGCCAACG CCTCTTGCAGCGCGGCGGCTTCGA AGCCGCCGCCCGGAGCTGCCCTTTCCTCTTCGGTGAAGTTTTTAAAAGCTG CTAAAGACTCGG +246 bp C N C N C N C N TG2 p65 Histones D • -760 bp • GGGTGATTTTGCCTTTGAGAGTCTGGATGAGAAATGCATGGTTAAAGGCAATTCCAGACAGGAAGAAAGGCAGAGA • AGAGGGTAGAAATGACCTCTGATTCTTGGGGCTGAGGGTTCCTAGAGCAAATGGCACAATGCCACGAGGCCCGATC • TATCCCTATGACGGAATCTAAGGTTTCAGCAAGTATCTGCTGGCTTGGTCATG -460 bp IκBα

  3. Fig. S3. TG2 and AR protein expression in selected prostate tumor samples (A). TG2 expression Is associated with increased accumulation of HIF-1α protein in prostate cancer cells (B) TG2 AR A B LnCap-TG2 LnCap-EV PC3  HIF-1α  TG2  β-actin

  4. Table S1 – Primers used to amplify various transcripts Gene designationPrimer Sequence (5’-3’) AR F: TTGGATGGCTCCAAATCAC R: GCAATGATACGATCG AGTTCC E-cadherin F: TCCCATCAGCTGCCCAGA A A R:TGACTCCTGTGTTCCTGT TA N-cadherin F: ACAGTGGCCACCTACAAAGG R: CCGAGATGGGGTTGATAATG VimentinF: GAG AACTTTGCCGTTGAA GC R: GCTTCCTGTAGGTGGCAATC FibronectinF: CAGTGGGAGACCTCGAGAAG R:TCCCTCGGAACATCAGAAAC ZEB1 F: CTGAAGAGGACCAGAGGCAG R: CCCAGAACTGCGTCACATGTC ZEB2 F: AAGTACCGCCACGAGAAGAA R: TTGTTTCTCATTCGGCCATT Snail F: TTCTTCTGCGCTACTGCTGCG R: GGGCAGGTATGGAGAGGAAGA TG2 F: TATGGCCAGTGCTGGGTCTTCGCC R: GGCTCCAGGGTTAGGTTGAGCAGG GAPDH F: ACCACAGTCCATGCCATCAC R: TCCACCACCCTGTTGCTGTA

More Related