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MPP OUTSIDE REQUEST FORM Written: 1/20/12 Requestor Reviewed:

MPP OUTSIDE REQUEST FORM Written: 1/20/12 Requestor Reviewed: Requestor(s): Dennis Winge Execs Reviewed: Institution: U. Utah Outcome : Request Title: yeast mitochondrial protein SDH1.

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MPP OUTSIDE REQUEST FORM Written: 1/20/12 Requestor Reviewed:

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  1. MPP OUTSIDE REQUEST FORMWritten: 1/20/12 Requestor Reviewed: Requestor(s):Dennis WingeExecs Reviewed: Institution:U. Utah Outcome: Request Title: yeast mitochondrial protein SDH1

  2. >tr|E9PBJ5|E9PBJ5_HUMAN Uncharacterized protein OS=Homo sapiens GN=SDHA PE=4 SV=1 SDHAuniprot >NP_004159, succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial [Homo sapiens], SDHA from mitocarta clone collection, BC001380, 1995 nt=665 aa, entrez 6389, this is 664aa uniprot MSGVRGLSRLLSARRLALAKAWPTVLQTGTRGFHFTVDGNKRASAKVSDSISAQYPVVDH 60 NP_004159 MSGVRGLSRLLSARRLALAKAWPTVLQTGTRGFHFTVDGNKRASAKVSDSISAQYPVVDH 60 ************************************************************ uniprot EFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKLFPTRSHTVAAQ---------------- 104 NP_004159 EFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKLFPTRSHTVAAQGGINAALGNMEEDNWR 120 ******************************************** uniprot --------------------------------LENYGMPFSRTEDGKIYQRAFGGQSLKF 132 NP_004159 WHFYDTVKGSDWLGDQDAIHYMTEQAPAAVVELENYGMPFSRTEDGKIYQRAFGGQSLKF 180 **************************** uniprot GKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIE 192 NP_004159 GKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIE 240 ************************************************************ uniprot DGSIHRIRAKNTVVATGGYGRTYFSCTSAHTSTGDGTAMITRAGLPCQDLEFVQFHPTGI 252 NP_004159 DGSIHRIRAKNTVVATGGYGRTYFSCTSAHTSTGDGTAMITRAGLPCQDLEFVQFHPTGI 300 ************************************************************ uniprot YGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCGPE 312 NP_004159 YGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCGPE 360 ************************************************************ uniprot KDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQ 372 NP_004159 KDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQ 420 ************************************************************ uniprot VLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDK 432 NP_004159 VLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDK 480 ************************************************************ uniprot VPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKIS 492 NP_004159 VPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKIS 540 ************************************************************ uniprot KLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVR 552 NP_004159 KLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVR 600 ************************************************************ uniprot IDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPA 612 NP_004159 IDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPA 660 ************************************************************ uniprot IRSY 616 NP_004159 IRSY 664 **** so the ncbi model, and the clone in the mootha lab collection, contain an additional internal seq not in the uniprot model. Will upload the ncbi seq.

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