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1h at 37°C

HIV-1 adsorption. 4 washes. Overnight at 4°C. Triton. C14 molecule. Poly-L-lysine wells. Medium. 1h at 37°C. 4 washes. Addition of PBLs. Culture for 6 days. * *. HIV-production (dosage of p24 antigen). * *. 1800. 1600. 1400. 1200. Medium. p24 (pg/ml). 1000. C14 (10  M). 800.

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1h at 37°C

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  1. HIV-1 adsorption 4 washes Overnight at 4°C Triton C14 molecule Poly-L-lysine wells Medium 1h at 37°C 4 washes Addition of PBLs Culture for 6 days * * HIV-production (dosage of p24 antigen) * * 1800 1600 1400 1200 Medium p24 (pg/ml) 1000 C14 (10 M) 800 C14 (20 M) 600 400 200 0 HIVNDKl Triton HIVBa-Ll

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