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Drosophila melanogaster Staining Lab

Drosophila melanogaster Staining Lab. Matthew Lee Keenan Mahan. Basic Drosophila melanogaster larva Anatomy. Image courtesy of Morgellons - UK. Adult Fly-C62.8LZ Type. The lower abdomen of the fly was completely marked with blue stain.

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Drosophila melanogaster Staining Lab

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  1. Drosophila melanogasterStaining Lab Matthew Lee Keenan Mahan

  2. Basic Drosophila melanogasterlarvaAnatomy Image courtesy of Morgellons-UK

  3. Adult Fly-C62.8LZ Type • The lower abdomen of the fly was completely marked with blue stain. • We believe that this shows the neural pathways within the leg muscles of the fly. • The diagram next to our images shows the muscle development that occurs within Drosophila.

  4. Adult Fly-C62.8LZ Type Image courtesy of PhysOrg.com

  5. Larva-C62.8LZ Type • In adult flies the C62.8LZ Type had highlighted regions in the abdomen that linked to the legs at one point. • In the larva the C62.8LZ has the stain in areas where the legs begin to develop as well as in what appears to be a neural pathway from the head of the larva to the anus. • The C62.8LZ Type is stained mostly in motor neuron pathways in the legs and in some other areas of the body in both the adult fly and larva.

  6. Larva-C62.8LZ Type Image courtesy of Dr. Brian Staveley

  7. Larva-en Lac Z Type • For en Lac Z Type larva the midintenstine, or midgut, was the part most stained. • When compared to the picture on the right of our pictures one can see how in both pictures the larva’s intestines are exposed and stained a blue/purple color.

  8. Larva-en Lac Z Type Image courtesy of IndranilMukhopadhyay, Daya Krishna Saxena, and DebapratimKarChowdhuri

  9. Larva-C362.14 UAS1 Type • The part of the larva stained for this type appears to be the salivary glands. • On each side of the larva there is a sac that is clearly stained in blue, and that sac is located at the same region as the salivary glands in Drosophila. • When compared to the highlighted salivary glands in the three larvae in the picture on the right, it appears as though salivary glands are clearly stained in both pictures.

  10. Larva-C362.14 UAS1 Type Image courtesy of TriTech Research

  11. Image Sources • "BIOL3530: Developmental Biology, Drosophila Development." Memorial University. Web. 07 Dec. 2010. <http://www.mun.ca/biology/desmid/brian/BIOL3530/DB_02/DBNDros.html>. • "Close Associations: Micro-Myiasis & Morgellons Disease." Morgellons UK - Campaign 2010 - Raising Awareness to the Public and Professionals. Web. 07 Dec. 2010. <http://www.morgellonsuk.org.uk/micromyiasis.htm>. • "Drosophila Vials, Bottles, Incubators, Fly Pads, CO2 Anasthesia Stations and Other Products - Tritech Research, Inc." Specialists in Microinjectors, Incubators and Electroporators - Tritech Research, Inc. Web. 07 Dec. 2010. <http://www.tritechresearch.com/dros.html>. • Mukhopadhyay, Indranil, Daya Krishna Saxena, and DebapratimKarChowdhuri. "Hazardous Effects of Effluent from the Chrome Plating Industry: 70 KDa Heat Shock Protein Expression as a Marker of Cellular Damage in Transgenic Drosophila Melanogaster (hsp70-lacZ)." EHP Online: Home of Environmental Health Perspectives. Web. 07 Dec. 2010. <http://ehpnet1.niehs.nih.gov/members/2003/6339/6339.html>. • Staveley, Brian. "Fruit Flies -- A Model for Bodybuilders." PhysOrg.com - Science News, Technology, Physics, Nanotechnology, Space Science, Earth Science, Medicine. Web. 07 Dec. 2010. <http://www.physorg.com/news187540044.html>.

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