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MFI 1.2 ±0.3

(i) Freshly isolated uraemic monocytes. CD40*. CD80*. CD86*. MFI 1.4 ±0.4. MFI 1.2 ±0.3. MFI 1.3 ±0.5. (ii) LPS-stimulated uraemic monocytes in complete medium. MFI 3.3 ±1.1. MFI 1.9 ±0.5. MFI 2.2 ±0.7. (iii) LPS-stimulated uraemic monocytes in autologous uraemic medium.

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MFI 1.2 ±0.3

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  1. (i) Freshly isolated uraemic monocytes CD40* CD80* CD86* MFI 1.4±0.4 MFI 1.2±0.3 MFI 1.3±0.5 (ii) LPS-stimulated uraemic monocytes in complete medium MFI 3.3±1.1 MFI 1.9±0.5 MFI 2.2±0.7 (iii) LPS-stimulated uraemic monocytes in autologous uraemic medium MFI 0.0±0.0 MFI 0.0±0.0 MFI 0.0±0.0 (iv) LPS-stimulated normal monocytes in complete medium MFI 8.7±1.6 MFI 2.7±0.6 MFI 3.3±0.9

  2. [3H] thymidine incorporation (cpm) *p<0.0001 Monocyte numbers Results expressed as mean±SD, Mann Whitney U *p<0.0001

  3. LPS-stimulated normal monocyte-derived DC in complete medium CD14 CD40 CD80 CD83 CD86* MFI 0.2±0.2 MFI 104.2±54.3 MFI 7.5±3.6 MFI 3.7±2.8 MFI 23.1±4.3 LPS-stimulated normalmonocyte-derived DC cultured in uraemic medium (CAPD) MFI 0.2±0.1 MFI 111.5±24.6 MFI 3.2±2.6 MFI 0.8±0.5 MFI 11.1±4.7 LPS-stimulated normalmonocyte-derived DC cultured in uraemic medium (CRF) MFI 0.8±0.2 MFI 110.2±64.3 MFI 4.3±2.7 MFI 1.4±0.9 MFI 15.2±7.2

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