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The Treatment and Prevention of Dental Caries

The Treatment and Prevention of Dental Caries. By: Scott Karas. Overview. Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses. Dental Caries. What are they? Cavities How are they formed? Streptococci mutans How to prevent them?

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The Treatment and Prevention of Dental Caries

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  1. The Treatment and Prevention of Dental Caries By: Scott Karas

  2. Overview • Dental Caries • Key Enzymes/Antibodies • Experiment Overview • Methods • Results • Discussion • Potential Uses

  3. Dental Caries • What are they? • Cavities • How are they formed? • Streptococci mutans • How to prevent them? • Brush your teeth • Vaccine?

  4. Key Enzymes/Antibodies • GTF- Glucosiltransferase • GbpB- Glucan binding protein B • CAT- Catalytic Domain • GTF epitope • GLU- Glucan binding domain • SYI- peptide complex • GbpBepitope • WIS-Water insoluble glucan

  5. Experiment Overview • Purpose of this experiment • Bacteria used • Streptococci mutans • Streptococci sobrinus • Synthetic peptides • Three experiments • 24-37 day old rats • Measured the levels of antibodies • Measured dental caries

  6. Methods • Peptides • Three monoepitopic • Two diepitopic • Proteins • GbpB from S. mutans • GTP from S. sobrinus • ELISA • Measured IgA and IgG antibody levels

  7. Methods • Experiment 1 • 40 female rats • 6 groups of 7 • Injected subconsciously • Experiment 2- S. mutans • 48 female rats • 4 groups of 12 in a cage • Injected subconsciously • Asphyxiated • Cleaned with beetles and ethanol

  8. Methods • Experiment 3- S. sobrinus • Identical to Experiment 2 • Streptomycin-resistant S. sobrinus • Bacterial Recovery • ELISA • Cotton swab • Carie assessment

  9. Potential Uses • Human Trials • Put in cows milk or eggs • Treatment for children or infants • “Window of Infectivity” • Treat young mothers • Breast feeding

  10. Summary • Dental Caries • Methods • Results • Potential Uses

  11. Summary • Dental Caries • Key Enzymes/Antibodies • Experiment Overview • Methods • Results • Discussion • Potential Uses

  12. References • Smith, Daniel J., William F. King, Joy Rivero, and Martin A. Taubman. "Immunological and Protective Effects of Diepitopic Subunit Dental Caries Vaccines." Infection and Immunity 73.5 (2005): 2797-804. Print. • Russell, Michael W., Noel K. Childers, Suzanne M. Michalek, Daniel J. Smith, and Martin A. Taubman. "A Caries Vaccine?" ProQuest Nursing & Allied Health Source 38.3 (2004): 230-35. Print.

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