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“Overview of Utz Lab” ITI Retreat at Chaminade PJ Utz, MD Associate Professor of Medicine March 11, 2007

“Overview of Utz Lab” ITI Retreat at Chaminade PJ Utz, MD Associate Professor of Medicine March 11, 2007. Main Research Goals: Technology Development for Analysis of IMIDs: Autoantibody Profiling FACS-based Technologies Reverse Phase Arrays Micro and Nano Fluidics

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“Overview of Utz Lab” ITI Retreat at Chaminade PJ Utz, MD Associate Professor of Medicine March 11, 2007

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  1. “Overview of Utz Lab” ITI Retreat at Chaminade PJ Utz, MD Associate Professor of Medicine March 11, 2007

  2. Main Research Goals: Technology Development for Analysis of IMIDs: Autoantibody Profiling FACS-based Technologies Reverse Phase Arrays Micro and Nano Fluidics Novel Detection (Carbon Nanotubes) Bioinformatics Antigen-Specific Therapy, Oligos Mouse Models of SLE, T1D Study of Human Materials

  3. Disease Interests: CTDs T1D IBD Sclerosing Cholangitis Primary Biliary Cirrhosis ANCA+ Vasculitis Cancer Atherosclerosis Transplantation Pediatric SLE Education

  4. Baechler et al., PNAS, 2003, 100:2610

  5. Bead-Based Multiplexed Cytokine Assay Adapted from Luminex website tutorial

  6. Bauer et al., PLOS Med, 2006, 3:2274

  7. Interferon and TLR Signaling IFN-I (IFN-a, IFN-b) IFNAR1 IFNAR2 JAK1 TYK2 ssRNA P P STAT1 STAT1 STAT2 STAT2 TLR 7 dsDNA TLR 9 IRF-9 IRF-9 IFN-b, IFN-a isotypes IFN inducible genes ISRE IRFs

  8. Produce arrays using a robotic microarrayer

  9. ALL MICE WT vs. PBS WT vs. IRF-9 WT vs. STAT-1

  10. Summary: Interferon Signaling in Mice • B cells from IRF9-/- and IFNAR2-/- mice express lower basal levels of TLR7 and fail to upregulate TLR7 in response to IFN-I. • Novel role for IFN-I in TLR-7 specific B cell responses and production of anti-RNP antibodies in vivo.

  11. IFN high/lowDNA-associated Antigens

  12. IFN high/lowRNA-associated Antigens

  13. Reverse Phase Protein Lysate Microarrays

  14. Cell Signaling in T Cell Leukemia Chan et al. , Blood, In Press

  15. Phospho-Protein Flow Cytometry Perez and Nolan, Nature Biotechnology, 2002, 20:155

  16. Signal Transduction Biosignatures Stratify AML Patients into Clinical Subgroups Irish et al, Cell, 2004, 118:217

  17. Development of New Therapies Analysis of Existing Therapies Bench Bedside Bench Animal Patient Immune Models Materials Monitoring Core

  18. Patient Sample Acquisition IRB 5 Clinics (Ritu Gupta) Trials Interdisciplinary Clinical Data, Outcome Measures Processing and Freezing Future Staff Additional Clinics, Backup New Clinics Processing by IMC Trials in Other Disciplines

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