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Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices. Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in

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Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

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  1. Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in Novel Advanced Materials and Processing with Applications in Biomedical, Electrical and Chemical Engineering

  2. Objective • Design localized and automated delivery system for microfluidic brain slice devices (μBSDs) • Delivers chemicals/drugs and gases • Automatically mixes oxygen • Characterize delivery of chemicals through tissue Image taken from Mohammed, J, Caicedo, H, Fall, C, & Eddington, D. (2008). Microfluidic add-on for standard electrophysiology chambers. Lab Chip, 8, 1048-1055.

  3. Motivation • μBSDs allow in vitro study of: • Neurogenesis • Synaptogenesis • Regeneration • Protein expression • Responses to physical trauma Image taken from http://www.koki.hu/cdnb/research/research.html Rambani, K, Vukasinovic, J, Glezer, A, & Potter, S. (2009). Culturing thick brain slices: an interstitial 3d microperfusion system for enhanced viability. Journal of Neuroscience Methods, 180, 243-254.

  4. Relevance • Chemical Delivery • Spatial resolution • Current systems affect entire slice with drug • Desire stimulation of specific areas • Oxygen Delivery • Automatic Mixing • Saves money • Pre-mixing is not required • Automation • Multi-tasking Image modified fromhttp://www.imagingeconomics.com/issues/articles/MI_2006-08_01.asp

  5. Oxygen Mixing and DeliveryExperimental Set-Up • Oxygen concentration measured by a NeoFox Fiber Optic Oxygen (FOXY) sensor Valves Gas Feed Lines: 0% and 21% Y Connector Output Tube

  6. Oxygen Mixing and DeliveryOutput

  7. Oxygen Mixing and DeliveryGraphical User Interface (GUI)

  8. Delivery CharacterizationμBSD Design Reservoir T Channel Delivery Channel

  9. Delivery CharacterizationExperimental Set-up DI Water Lines T Channel Valve μBSD Vacuum Line

  10. Delivery CharacterizationNo Tissue Delivery 10ms 15ms 20ms 25ms 2x5ms 3x5ms 4x5ms 5x5ms

  11. Delivery CharacterizationLinear Relationships: No Tissue 860 Width (µm) 1320 Width (µm)

  12. Delivery CharacterizationLinear Relationships: With Tissue Full Dose Chemical Delivery Maximum Intensities Intensity Intensity 4 2 0 2 6 Number of 5ms Pulses Time (min) Pulsing Dose Chemical Delivery Maximum Intensities Intensity Intensity 30 20 2 0 10 Time (min) Valve Open Time (ms)

  13. Delivery CharacterizationGUI

  14. Conclusions • Oxygen Delivery • Precise and accurate results • Reliable oxygen switching • Chemical Delivery • Demonstrated linear relationship • Area of effect: ~2mm x 2mm • Selective and independent valve control

  15. Acknowledgements • Funding • NSF-REU • DoD-ASSURE • EEC-NSF Grant # 0755115 • REU Directors • Christos G. Takoudis, Ph.D. • Greg Jursich, Ph.D. • Research Advisor • David Thomas Eddington, Ph.D. • Mentors • Alexander Blake, Ph.D. • Gerardo Mauleon

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