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UMM AL-QURA UNIVERSITY Faculty of Medicine Dep. Of BIOCHEMISTRY

بسم الله الرحمن الرحيم. UMM AL-QURA UNIVERSITY Faculty of Medicine Dep. Of BIOCHEMISTRY. ACID-BASE TITRATION. To determine the concentration of a given acid by its titration against alkali. OBJECTIVES:.

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UMM AL-QURA UNIVERSITY Faculty of Medicine Dep. Of BIOCHEMISTRY

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  1. بسم الله الرحمن الرحيم UMM AL-QURA UNIVERSITYFaculty of MedicineDep. Of BIOCHEMISTRY

  2. ACID-BASE TITRATION

  3. To determine the concentration of a given acid by its titration against alkali. OBJECTIVES:

  4. An acidis a substance that increases the concentration of H3O+ (hydronium ion) in aqueous solution. • A base: is a substance that decreases the concentration of H3O+ in aqueous solution. • A decrease in [H3O+] requires an increase in [OH-], so we can define the base as a substance that increase the concentration of OH- in aqueous solution. • H+ is called a proton, because a proton is all that remains when a hydrogen atom loses its electron. • Hydronium ion (H3O+) is a combination of H+ with H2O. What are acids and bases?

  5. Bronsted and Lowry definition of acids and bases • An acid is a proton donor • A base is a proton acceptor. • Example • HCl is an acid because it donates a proton to H2O to form H3O+ • HCl(l) + H2O H3O+(aq) + Cl-(aq) • Salts: • - Any ionic solid such as ammonium chloride is called salt. It can be thought of as the product of an acid base reaction. • Most salts are strong electrolytes, i.e. they dissociate almost completely into their component ions when dissolved in water • NH4+Cl- NH4+ + Cl-

  6. Astandard solution (standard titrant)is a reagent of known concentration that is used to carry out a titrimetric analysis • Titration: is a procedure performed by adding a standard solution from a buret or other liquid-dispensing devices to a solution of the analyte until the reaction between the two is judged complete. • Equivalence pointin titration is reached when the amount of added titrant is chemically equivalent to the amount of analyte in the sample • E.g. AgNO3 + Cl- AgCl mol mol • (Equivalent points can’t be determined experimentally) • End pointis the point in a titration when physical change occurs that is associated with the condition of chemical equivalence. • Indicator: a chemical compound that change it’s color or other physical property at or near the equivalence point. Definitions:

  7. ABDULLATIF TAHA ABDULLAMSc. BIOCHEMISTRY سبحانك لا علم لنا إلا ما علمتنا * إنك أنت العليم الحكيم 0 QUESTIONS?

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